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dc.contributor.author
Santana, Hector
dc.contributor.author
Avila, Cesar Luis
dc.contributor.author
Cabrera, Ingrid
dc.contributor.author
Paez, Rolando
dc.contributor.author
Falcon, Viviana
dc.contributor.author
Pessoa, Adalberto
dc.contributor.author
Ventosa, Nora
dc.contributor.author
Veciana, Jaume
dc.contributor.author
Itri, Rosangela
dc.contributor.author
Barbosa, Leandro Ramos Souza
dc.date.available
2017-12-15T20:20:32Z
dc.date.issued
2014-11
dc.identifier.citation
Santana, Hector; Avila, Cesar Luis; Cabrera, Ingrid; Paez, Rolando; Falcon, Viviana; et al.; How does growth hormone releasing hexapeptide self-assemble in nanotubes?; Royal Society of Chemistry; Soft Matter; 10; 46; 11-2014; 9260-9269
dc.identifier.issn
1744-683X
dc.identifier.uri
http://hdl.handle.net/11336/30817
dc.description.abstract
Growth hormone releasing peptide, GHRP-6, a hexapeptide (His-(D-Trp)-Ala-Trp-(D-Phe)-Lys-NH2, MW = 872.44 Da) that belongs to a class of synthetic growth hormone secretagogues, can stimulate growth hormone secretion from somatotrophs in several species including humans. In the present study, we demonstrate that GHRP-6 dispersed in aqueous solution, at pH 7.0, room temperature of 22 °C, is able to form long nanotubes, which is evidenced by combining small angle X-ray scattering (SAXS), transmission electron microscopy and molecular dynamics simulation results. Such nanotubes possess inner and outer cross-sections equal to 6.7(2) nm and 13.4(5) nm, respectively. The mechanism of peptide self-assembly was determined by molecular dynamics simulations revealing that the peptides self-assemble like amphiphilic molecules in aqueous solution in a partially interdigitated structure. In this case, the position of the positively charged amino terminus is located at the peptide–water interface, whereas the neutral NH2-capped carboxy terminus remains buried at the hydrophobic core. In contrast, the long side chain of Lys-6 stretches out of the hydrophobic core positioning its positive charge near the cylinder surface. The peptide configuration in the nanotube wall comes from the interplay between the hydrophobic interactions of the aromatic side chains of GHRP-6 and the electrostatic repulsion of its cationic charges. On increasing the peptide concentration, the long nanotubes self-arrange in solution displaying a bi-dimensional hexagonal-like packing in the SAXS curves, with a center-to-center distance of ∼15 nm. Further, we also show that the nanostructure formed in solution is quite stable and is preserved following transfer to a solid support.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Royal Society of Chemistry
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Peptide
dc.subject
Self-Aggregation
dc.subject
Nanotubes
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
How does growth hormone releasing hexapeptide self-assemble in nanotubes?
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-12-11T19:23:31Z
dc.journal.volume
10
dc.journal.number
46
dc.journal.pagination
9260-9269
dc.journal.pais
Reino Unido
dc.description.fil
Fil: Santana, Hector. Center for Genetic Engineering and Biotechnology; Cuba
dc.description.fil
Fil: Avila, Cesar Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
dc.description.fil
Fil: Cabrera, Ingrid. Consejo Superior de Investigaciones Científicas. Instituto de Ciencia de Los Materiales de Barcelona; España. Centro de Investigación Biomédica en Red; España
dc.description.fil
Fil: Paez, Rolando. Center for Genetic Engineering and Biotechnology; Cuba
dc.description.fil
Fil: Falcon, Viviana. Center for Genetic Engineering and Biotechnology; Cuba
dc.description.fil
Fil: Pessoa, Adalberto. Universidade de Sao Paulo; Brasil
dc.description.fil
Fil: Ventosa, Nora. Centro de Investigación Biomédica en Red; España. Consejo Superior de Investigaciones Científicas. Instituto de Ciencia de Los Materiales de Barcelona; España
dc.description.fil
Fil: Veciana, Jaume. Centro de Investigación Biomédica en Red; España. Consejo Superior de Investigaciones Científicas. Instituto de Ciencia de Los Materiales de Barcelona; España
dc.description.fil
Fil: Itri, Rosangela. Universidade de Sao Paulo; Brasil
dc.description.fil
Fil: Barbosa, Leandro Ramos Souza. Universidade de Sao Paulo; Brasil
dc.journal.title
Soft Matter
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1039/c4sm01693a
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2014/SM/C4SM01693A#!divAbstract
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