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Artículo

Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction

Astort, FranciscoIcon ; Mercau, María ElisaIcon ; Giordanino, E. F.; Degese, María SolIcon ; Caldareri, Lilian Julia; Coso, Omar AdrianIcon ; Cymeryng, Cora BetrizIcon
Fecha de publicación: 12/2013
Editorial: Elsevier Inc
Revista: Nitric Oxide-biology and Chemistry
ISSN: 1089-8603
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

Induction of microsomal heme oxygenase 1 (HO-1) activity is considered a cytoprotective mechanism in different cell types. In adrenal cells, HO-1 induction by ACTH exerts a modulatory effect on steroid production as well. As nitric oxide (NO) has been also regarded as an autocrine/paracrine modulator of adrenal steroidogenesis we sought to study the effects of NO on the induction of HO-1 and the mechanism involved. We hereby analyzed the time and dose-dependent effect of a NO-donor (DETA/NO) on HO-1 induction in a murine adrenocortical cell line. We showed that this effect is mainly exerted at a transcriptional level as it is inhibited by actinomycin D and HO-1 mRNA degradation rates were not affected by DETA/NO treatment. HO-1 induction by NO does not appear to involve the generation of oxidative stress as it was not affected by antioxidant treatment. We also demonstrated that NO-treatment results in the nuclear translocation of the nuclear factor-erythroid 2-related factor (Nrf2), an effect that is attenuated by transfecting the cells with a dominant negative isoform of Nrf2. We finally show that the effects of the NO-donor are reproduced by a permeable analog of cGMP and that a soluble guanylate cyclase specific inhibitor blocked both the induction of HO-1 by NO and the nuclear translocation of Nrf2.
Palabras clave: Hemooxigenasa , Oxido Nítrico , Corteza Adrenal , Estrés Oxidativo
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/30816
DOI: http://dx.doi.org/10.1016/j.niox.2013.12.006
URL: http://www.sciencedirect.com/science/article/pii/S1089860313003455
Colecciones
Articulos(CEFYBO)
Articulos de CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Articulos(IFIBYNE)
Articulos de INST.DE FISIOL., BIOL.MOLECULAR Y NEUROCIENCIAS
Citación
Astort, Francisco; Mercau, María Elisa; Giordanino, E. F.; Degese, María Sol; Caldareri, Lilian Julia; et al.; Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction; Elsevier Inc; Nitric Oxide-biology and Chemistry; 37; 12-2013; 1-10
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