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dc.contributor.author
Gazzaniga, Silvina Noemí
dc.contributor.author
Bravo, Alicia I.
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Guglielmotti, Angelo
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Rooijen, Nico van
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Maschi, Fabricio
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Vecchi, Annunciata
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Mantovani, Alberto
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Mordoh, Jose
dc.contributor.author
Wainstok, Rosa
dc.date.available
2017-12-15T20:16:23Z
dc.date.issued
2007
dc.identifier.citation
Gazzaniga, Silvina Noemí; Bravo, Alicia I.; Guglielmotti, Angelo; Rooijen, Nico van; Maschi, Fabricio; et al.; Targeting Tumor-Associated Macrophages and Inhibition of MCP-1 Reduce Angiogenesis and Tumor Growth in a Human Melanoma Xenograft; Elsevier; Journal Of Investigative Dermatology; 127; 8; 2007; 2031-2041
dc.identifier.issn
0022-202X
dc.identifier.uri
http://hdl.handle.net/11336/30811
dc.description.abstract
Chemokines such as monocyte chemoattractant protein (MCP)-1 are key agonists that attract macrophages to tumors. In melanoma, it has been previously shown that variable levels of MCP-1/CCL2 appear to correlate with infiltrating macrophages and tumor fate, with low to intermediate levels of the chemokine contributing to melanoma development. To work under such conditions, a poorly tumorigenic human melanoma cell line was transfected with an expression vector encoding MCP-1. We found that M2 macrophages are associated to MCP-1+ tumors, triggering a profuse vascular network. To target the protumoral macrophages recruitment and reverting tumor growth promotion, clodronate-laden liposomes (Clod-Lip) or bindarit were administered to melanoma-bearing mice. Macrophage depletion after Clod-Lip treatment induced development of smaller tumors than in untreated mice. Immunohistochemical analysis with an anti-CD31 antibody revealed scarce vascular structures mainly characterized by narrow vascular lights. Pharmacological inhibition of MCP-1 with bindarit also reduced tumor growth and macrophage recruitment, rendering necrotic tumor masses. We suggest that bindarit or Clod-Lip abrogates protumoral-associated macrophages in human melanoma xenografts and could be considered as complementary approaches to antiangiogenic therapy.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject.classification
Medicina Critica y de Emergencia
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Medicina Clínica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Targeting Tumor-Associated Macrophages and Inhibition of MCP-1 Reduce Angiogenesis and Tumor Growth in a Human Melanoma Xenograft
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-11-03T20:23:03Z
dc.journal.volume
127
dc.journal.number
8
dc.journal.pagination
2031-2041
dc.journal.pais
Países Bajos
dc.description.fil
Fil: Gazzaniga, Silvina Noemí. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
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Fil: Bravo, Alicia I.. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos "Eva Perón"; Argentina
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Fil: Guglielmotti, Angelo. Angelini Farmaceutici; Italia
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Fil: Rooijen, Nico van. No especifica;
dc.description.fil
Fil: Maschi, Fabricio. Universidad Nacional de La Plata; Argentina
dc.description.fil
Fil: Vecchi, Annunciata. Istituto Clinico Humanitas; Italia
dc.description.fil
Fil: Mantovani, Alberto. Istituto Clinico Humanitas; Italia
dc.description.fil
Fil: Mordoh, Jose. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Wainstok, Rosa. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Journal Of Investigative Dermatology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1038/sj.jid.5700827
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022202X15335144
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