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dc.contributor.author
Accialini, Paula Lucia
dc.contributor.author
Hernandez, Silvia Fátima
dc.contributor.author
Bas, Diana Ester
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Pazos Maidana, María Camila
dc.contributor.author
Irusta, Griselda
dc.contributor.author
Abramovich, Dalhia Nurit
dc.contributor.author
Tesone, Marta
dc.date.available
2015-12-18T18:48:54Z
dc.date.issued
2015-01
dc.identifier.citation
Accialini, Paula Lucia; Hernandez, Silvia Fátima; Bas, Diana Ester; Pazos Maidana, María Camila; Irusta, Griselda; et al.; A link between Notch and progesterone maintains the functionality of the rat corpus luteum; Bioscientifica; Reproduction; 149; 1; 1-2015; 1-10
dc.identifier.issn
1470-1626
dc.identifier.uri
http://hdl.handle.net/11336/3061
dc.description.abstract
In this study, we investigated the interaction between the Notch pathway and progesterone to maintain the functionality of the corpus luteum(CL).WhenNotch signaling is activated, the g-secretase complex releases the active intracellular domains (NICD) of their receptors, which exert survival effects. We designed studies to analyze whether the in vitro inhibition of Notch affects progesterone production, steroidogenic regulators, apoptotic parameters, and signaling transduction pathways in the cultures of CL isolated from pregnant and superovulated rats. We detected a decrease in progesterone production when corpora lutea (CL) were incubated with N-(N-(3,5- difluorophenacetyl-L-alanyl))-S-phenylglycine t-butyl ester (DAPT), a g-secretase inhibitor. This effect could be in part due to the decrease detected in the CL protein levels of P450scc because STAR and 3b-hydroxysteroid dehydrogenase were not affected by Notch inhibition. Besides, the addition of aminoglutethimide to the CL culture medium decreased NICD of NOTCH1. We observed an increase in the expression of active CASPASE3 (CASP3) after inhibition by Notch,whichwas reversed by the presence of progesterone. The BAX:BCLXL ratio was increased in CL treated with DAPT and the presence of progesterone reversed this effect. In addition, phosphorylation of AKTwas inhibited inCL treated withDAPT, but had no effect on ERK activation. To demonstrate that the action ofDAPTis specifically related with the inhibition of Notch, CLswere incubated with DLL4 antibody and a decrease in progesterone production was detected. These results suggest the existence of a novel link between progesterone and the Notch signaling pathway to maintain the functionality of the CL.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Bioscientifica
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Notch
dc.subject
Ovary
dc.subject
Corpus Luteum
dc.subject
Progesterone
dc.subject.classification
Bioquímica y Biología Molecular
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Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
A link between Notch and progesterone maintains the functionality of the rat corpus luteum
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.identifier.eissn
1741-7899
dc.journal.volume
149
dc.journal.number
1
dc.journal.pagination
1-10
dc.journal.pais
Reino Unido
dc.journal.ciudad
Bristol
dc.description.fil
Fil: Accialini, Paula Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Hernandez, Silvia Fátima. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Bas, Diana Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Pazos Maidana, María Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Irusta, Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Abramovich, Dalhia Nurit. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Tesone, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.journal.title
Reproduction
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/149/1/1.abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/doi:10.1530/REP-14-0449
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