Artículo
Rat liver antioxidant response to iron and copper overloads
Musacco Sebio, Rosario Natalia
; Saporito Magriñá, Christian Martín
; Semprine, Jimena Vanina
; Torti, Horacio Emilio; Ferrarotti, Nidia Fatima; Castro-Parodi, Mauricio Omar; Damiano, Alicia Ermelinda
; Boveris, Alberto Antonio
; Repetto, Marisa Gabriela
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Fecha de publicación:
04/2014
Editorial:
Elsevier
Revista:
Journal of Inorganic Biochemistry
ISSN:
0162-0134
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The rat liver antioxidant response to Fe and Cu overloads (0–60 mg/kg) was studied. Dose- and time-responses were determined and summarized by t1/2 and C50, the time and the liver metal content for half maximal oxidative responses. Liver GSH (reduced glutathione) and GSSG (glutathione disulfide) were determined. The GSH content and the GSH/GSSG ratio markedly decreased after Fe (58–66%) and Cu (79–81%) loads, with t1/2 of 4.0 and 2.0 h. The C50 were in a similar range for all the indicators (110–124 μg Fe/g and 40–50 μg Cu/g) and suggest a unique free-radical mediated process. Hydrophilic antioxidants markedly decreased after Fe and Cu (60–75%; t1/2: 4.5 and 4.0 h). Lipophilic antioxidants were also decreased (30–92%; t1/2: 7.0 and 5.5 h) after Fe and Cu. Superoxide dismutase (SOD) activities (Cu,Zn-SOD and Mn-SOD) and protein expression were adaptively increased after metal overloads (Cu,Zn-SOD: t1/2: 8–8.5 h and Mn-SOD: t1/2: 8.5–8.0 h). Catalase activity was increased after Fe (65%; t1/2: 8.5 h) and decreased after Cu (26%; t1/2: 8.0 h), whereas catalase expression was increased after Fe and decreased after Cu overloads. Glutathione peroxidase activity decreased after metal loads by 22–39% with a t1/2 of 4.5 h and with unchanged protein expression. GSH is the main and fastest responder antioxidant in Fe and Cu overloads. The results indicate that thiol (SH) content and antioxidant enzyme activities are central to the antioxidant defense in the oxidative stress and damage after Fe and Cu overloads.
Palabras clave:
Antioxidants
,
Liver Chemiluminescence
,
Copper
,
Glutathione
,
Iron
,
Oxidative Damage
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Musacco Sebio, Rosario Natalia; Saporito Magriñá, Christian Martín; Semprine, Jimena Vanina; Torti, Horacio Emilio; Ferrarotti, Nidia Fatima; et al.; Rat liver antioxidant response to iron and copper overloads; Elsevier; Journal of Inorganic Biochemistry; 137; 4-2014; 94-100
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