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dc.contributor.author
López Rodriguez, Maria Lorena
dc.contributor.author
Madrid, Rossana Elena
dc.contributor.author
Giacomelli, Carla Eugenia
dc.date.available
2017-12-12T18:02:55Z
dc.date.issued
2013-12
dc.identifier.citation
López Rodriguez, Maria Lorena; Madrid, Rossana Elena; Giacomelli, Carla Eugenia; The optimization of the culture medium to design Streptomyces sp. M7 based impedimetric biosensors; Elsevier Science; Sensors and Actuators B: Chemical; 193; 12-2013; 230-237
dc.identifier.issn
0925-4005
dc.identifier.uri
http://hdl.handle.net/11336/30312
dc.description.abstract
Electrochemical impedance has been widely used in microbiology in detection, quantifying and identifying microorganisms. Streptomyces strain M7 (SM7) is capable to grow in the presence of organochlorine pesticides (OCPs) as carbon source, using the amino acid asparagine as nitrogen source. This bacterial growth can be valuable and hence used in an impedimetric biosensor to detect and, eventually, quantify these pesticides. Since amino acids frequently used in culture media interfere in impedance determinations, this work presents an optimized design regarding the culture medium capabilities on the impedance response of SM7 activity, as a first stage toward the development of an impedimetric biosensor to detect OCPs. With such a purpose, SM7 growth in liquid or adhered to a solid media was measured by impedance in the presence of asparagine or (NH4)2SO4 as nitrogen source and glucose as a model carbon source. Impedimetric SM7 measurements were performed in the Quantibac instrument that can be employed to evaluate the metabolic behavior of microorganisms in different culture media using two fixed frequencies. We found that SM7 used asparagine as carbon source in the absence of glucose. Since this consumption strongly contributed to the impedance signal, this is not a suitable medium to be used with an impedimetric biosensor. On the other hand, (NH4)2SO4 appears as an ideal medium because it supports SM7 growth in liquid and solid media without interfering the impedimetric detection. This simple and cost-effective technique combined with a spore-based platform is a promising strategy to design OCPs bacteria-based biosensors.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Streptomyces
dc.subject
Carbon Source
dc.subject
Nitrogen Source
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Impedance
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Spore-Based Platform
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Organochlorine Pesticides
dc.subject.classification
Bioremediación, Diagnóstico Biotecnológico en Gestión Medioambiental
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Biotecnología del Medio Ambiente
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
The optimization of the culture medium to design Streptomyces sp. M7 based impedimetric biosensors
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-12-11T19:25:52Z
dc.journal.volume
193
dc.journal.pagination
230-237
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: López Rodriguez, Maria Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.description.fil
Fil: Madrid, Rossana Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
dc.description.fil
Fil: Giacomelli, Carla Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.journal.title
Sensors and Actuators B: Chemical
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.snb.2013.11.066
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400513014160
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