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dc.contributor.author
Pellegrini Malpiedi, Luciana  
dc.contributor.author
Nerli, Bibiana Beatriz  
dc.contributor.author
Abdalla, Dulcinéia Saes Parra  
dc.contributor.author
Pessoa, Adalberto Jr.  
dc.date.available
2017-12-12T17:18:39Z  
dc.date.issued
2014-05  
dc.identifier.citation
Pellegrini Malpiedi, Luciana; Nerli, Bibiana Beatriz; Abdalla, Dulcinéia Saes Parra; Pessoa, Adalberto Jr.; Assessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody Fragment; American Chemical Society; Biotechnology Progress; 30; 3; 5-2014; 554-561  
dc.identifier.issn
8756-7938  
dc.identifier.uri
http://hdl.handle.net/11336/30302  
dc.description.abstract
The effect of Triton X-114 on the physicochemical properties of a single-chain antibody fragment (scFv) has been studied. According to the far UV circular dichroism spectroscopy, the secondary structure of the recombinant antibody was not significantly affected by the presence of Triton. From the antibody tertiary structure analysis, it was found that the surfactant could be located around the tryptophan molecules accessible to the solvent, diminishing the polarity of its environment but maintaining most of the protein structure integrity. However, in certain conditions of high temperature and high concentration of denaturant molecules, the presence of TX could compromise the antibody fragment stability. These results represent a previous step in designing scFv purification protocols and should be considered prior to developing scFv liquid–liquid extraction procedures  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
American Chemical Society  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Nonionic Surfactant  
dc.subject
Single-Chain Antibody Fragment  
dc.subject
Spectroscopic Study  
dc.subject.classification
Otras Ciencias Químicas  
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Ciencias Químicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Assessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody Fragment  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2017-12-07T15:42:26Z  
dc.journal.volume
30  
dc.journal.number
3  
dc.journal.pagination
554-561  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Washington  
dc.description.fil
Fil: Pellegrini Malpiedi, Luciana. Universidade de Sao Paulo; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Quimicos Rosario. Universidad Nacional de Rosario. Facultad de Cs.bioquímicas y Farmaceuticas. Instituto de Procesos Biotecnológicos y Quimicos Rosario; Argentina  
dc.description.fil
Fil: Nerli, Bibiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Quimicos Rosario. Universidad Nacional de Rosario. Facultad de Cs.bioquímicas y Farmaceuticas. Instituto de Procesos Biotecnológicos y Quimicos Rosario; Argentina  
dc.description.fil
Fil: Abdalla, Dulcinéia Saes Parra. Universidade de Sao Paulo; Brasil  
dc.description.fil
Fil: Pessoa, Adalberto Jr.. Universidade de Sao Paulo; Brasil  
dc.journal.title
Biotechnology Progress  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/btpr.1882  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/btpr.1882/abstract