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dc.contributor.author
Pellegrini Malpiedi, Luciana
dc.contributor.author
Nerli, Bibiana Beatriz
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Abdalla, Dulcinéia Saes Parra
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Pessoa, Adalberto Jr.
dc.date.available
2017-12-12T17:18:39Z
dc.date.issued
2014-05
dc.identifier.citation
Pellegrini Malpiedi, Luciana; Nerli, Bibiana Beatriz; Abdalla, Dulcinéia Saes Parra; Pessoa, Adalberto Jr.; Assessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody Fragment; American Chemical Society; Biotechnology Progress; 30; 3; 5-2014; 554-561
dc.identifier.issn
8756-7938
dc.identifier.uri
http://hdl.handle.net/11336/30302
dc.description.abstract
The effect of Triton X-114 on the physicochemical properties of a single-chain antibody fragment (scFv) has been studied. According to the far UV circular dichroism spectroscopy, the secondary structure of the recombinant antibody was not significantly affected by the presence of Triton. From the antibody tertiary structure analysis, it was found that the surfactant could be located around the tryptophan molecules accessible to the solvent, diminishing the polarity of its environment but maintaining most of the protein structure integrity. However, in certain conditions of high temperature and high concentration of denaturant molecules, the presence of TX could compromise the antibody fragment stability. These results represent a previous step in designing scFv purification protocols and should be considered prior to developing scFv liquid–liquid extraction procedures
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Chemical Society
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Nonionic Surfactant
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Single-Chain Antibody Fragment
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Spectroscopic Study
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Otras Ciencias Químicas
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Assessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody Fragment
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-12-07T15:42:26Z
dc.journal.volume
30
dc.journal.number
3
dc.journal.pagination
554-561
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington
dc.description.fil
Fil: Pellegrini Malpiedi, Luciana. Universidade de Sao Paulo; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Quimicos Rosario. Universidad Nacional de Rosario. Facultad de Cs.bioquímicas y Farmaceuticas. Instituto de Procesos Biotecnológicos y Quimicos Rosario; Argentina
dc.description.fil
Fil: Nerli, Bibiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Quimicos Rosario. Universidad Nacional de Rosario. Facultad de Cs.bioquímicas y Farmaceuticas. Instituto de Procesos Biotecnológicos y Quimicos Rosario; Argentina
dc.description.fil
Fil: Abdalla, Dulcinéia Saes Parra. Universidade de Sao Paulo; Brasil
dc.description.fil
Fil: Pessoa, Adalberto Jr.. Universidade de Sao Paulo; Brasil
dc.journal.title
Biotechnology Progress
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/btpr.1882
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/btpr.1882/abstract
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