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dc.contributor.author Lattar, Santiago Martín
dc.contributor.author Noto Llana, Mariangeles
dc.contributor.author Denoël, Philippe
dc.contributor.author Germain, Sophie
dc.contributor.author Buzzola, Fernanda Roxana
dc.contributor.author Lee, Jean C.
dc.contributor.author Sordelli, Daniel Oscar
dc.date.available 2017-12-11T23:14:40Z
dc.date.issued 2014-01
dc.identifier.citation Lattar, Santiago Martín; Noto Llana, Mariangeles; Denoël, Philippe; Germain, Sophie; Buzzola, Fernanda Roxana; et al.; Protein Antigens Increase the Protective Efficacy of a Capsule-Based Vaccine against Staphylococcus aureus in a Rat Model of Osteomyelitis; American Society for Microbiology; Infection and Immunity; 82; 1; 1-2014; 83-91
dc.identifier.issn 0019-9567
dc.identifier.uri http://hdl.handle.net/11336/30231
dc.description.abstract Staphylococcus aureus is an invasive bacterial pathogen, and antibiotic resistance has impeded adequate control of infections caused by this microbe. Moreover, efforts to prevent human infections with single-component S. aureus vaccines have failed. In this study, we evaluated the protective efficacy in rats of vaccines containing both S. aureus capsular polysaccharides (CPs) and proteins. The serotypes 5 CP (CP5) and 8 CP (CP8) were conjugated to tetanus toxoid and administered to rats alone or together with domain A of clumping factor A (ClfA) or genetically detoxified alpha-toxin (dHla). The vaccines were delivered according to a preventive or a therapeutic regimen, and their protective efficacy was evaluated in a rat model of osteomyelitis. Addition of dHla (but not ClfA) to the CP5 or CP8 vaccine induced reductions in bacterial load and bone morphological changes compared with immunization with either conjugate vaccine alone. Both the prophylactic and therapeutic regimens were protective. Immunization with dHla together with a pneumococcal conjugate vaccine used as a control did not reduce staphylococcal osteomyelitis. The emergence of unencapsulated or small-colony variants during infection was negligible and similar for all of the vaccine groups. In conclusion, addition of dHla to a CP5 or CP8 conjugate vaccine enhanced its efficacy against S. aureus osteomyelitis, indicating that the inclusion of multiple antigens will likely enhance the efficacy of vaccines against both chronic and acute forms of staphylococcal disease.
dc.format application/pdf
dc.language.iso eng
dc.publisher American Society for Microbiology
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.subject STAPHYLOCOCCUS AUREUS
dc.subject CAPSULE
dc.subject VACCINE
dc.subject OSTEOMYELITIS
dc.subject.classification Inmunología
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Protein Antigens Increase the Protective Efficacy of a Capsule-Based Vaccine against Staphylococcus aureus in a Rat Model of Osteomyelitis
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-12-11T16:43:44Z
dc.journal.volume 82
dc.journal.number 1
dc.journal.pagination 83-91
dc.journal.pais Estados Unidos
dc.journal.ciudad Washington DC
dc.description.fil Fil: Lattar, Santiago Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
dc.description.fil Fil: Noto Llana, Mariangeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
dc.description.fil Fil: Denoël, Philippe. GlaxoSmithKline Vaccines; Bélgica
dc.description.fil Fil: Germain, Sophie. GlaxoSmithKline Vaccines; Bélgica
dc.description.fil Fil: Buzzola, Fernanda Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
dc.description.fil Fil: Lee, Jean C.. Harvard Medical School; Estados Unidos
dc.description.fil Fil: Sordelli, Daniel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
dc.journal.title Infection and Immunity
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3911846/
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://iai.asm.org/content/82/1/83.long
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/IAI.01050-13


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial 2.5 Unported (CC BY-NC 2.5)