Mostrar el registro sencillo del ítem

dc.contributor.author
Bermejo, Carolina Julieta
dc.contributor.author
Gatti, Ileana
dc.contributor.author
Caballero, Nancy
dc.contributor.author
Cravero, Vanina Pamela
dc.contributor.author
Martin, Eugenia Alejandra
dc.contributor.author
Cointry Peix, Enrique Luis
dc.date.available
2017-12-11T14:50:58Z
dc.date.issued
2014-05
dc.identifier.citation
Bermejo, Carolina Julieta; Gatti, Ileana; Caballero, Nancy; Cravero, Vanina Pamela; Martin, Eugenia Alejandra; et al.; Study of diversity in a set of lentil RILs using morphological and molecular markers; Southern Cross Publ; Australian Journal Of Crop Science; 8; 5; 5-2014; 689-696
dc.identifier.issn
1835-2693
dc.identifier.uri
http://hdl.handle.net/11336/30114
dc.description.abstract
We propose the use of SRAP and SSR markers along agronomic or morphological traits in genotypes’ differentiation during breeding programs. 25 F5 recombinant inbred lines (microsperma and macrosperma) with good quality characteristics and high yield and a tester were used. All materials were sowed in a randomized block design with 3 replications, at a density of 140 plants per m2 . Different morphological traits were recorded and analyzed using ANOVA. Euclidean distances were calculated and a cluster analysis and a principal components analysis (PCA) were carried out. A total of 20 out of 25 SRAP combinations and 9 out of 17 SSR were assayed on the basis of their high polymorphism. Genetic distances were calculated according to Dice’s similarity index and a dendrogram was performed. A comparison between morphological and molecular data was carried out through the procrustes generalized analysis. The analysis of variance for morphological traits showed significant differences between genotypes for all variables. The analysis of principal components determined that two PCs explained 75% of the total variation observed among the genotypes and the cluster analysis allowed the conformation of two principal groups. The first one included four varieties belonging to the microsperma type, whereas the second group included all the macrosperma type Rils. Both groups showed differences also in yield and plant height. Molecular analysis showed that 66% out of 273 bands are polymorphic fragments from SRAP markers, indicating a high genetic diversity among Rils. The number of alleles per SSR locus ranged from 3 (SSR 33) to 7 (SSR 156) showing also the variability present. Two exclusive SRAP fragments generated by the combination me5-em1 (230 bp) were found in microsperma group allowing early selection of this trait and all characters associated with it. The correlation between Dice similarity index and Euclidean distance matrices was 87% indicating an excellent consistency between both types of markers and suggested that both types of traits would provide similar estimates on the variability and would be useful to differentiate Rils with similar origin
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Southern Cross Publ
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Genetic Distance
dc.subject
Molecular Markers
dc.subject
Morphological Markers
dc.subject
Varieties Selection
dc.subject
Srap
dc.subject
Ssr
dc.subject.classification
Otras Biotecnología Agropecuaria
dc.subject.classification
Biotecnología Agropecuaria
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
Study of diversity in a set of lentil RILs using morphological and molecular markers
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-12-07T15:41:23Z
dc.identifier.eissn
1835-2707
dc.journal.volume
8
dc.journal.number
5
dc.journal.pagination
689-696
dc.journal.pais
Australia
dc.journal.ciudad
Lismore
dc.description.fil
Fil: Bermejo, Carolina Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Gatti, Ileana. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Producción Vegetal. Cátedra de Mejoramiento Vegetal y Prod.de Semillas; Argentina
dc.description.fil
Fil: Caballero, Nancy. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Producción Vegetal. Cátedra de Mejoramiento Vegetal y Prod.de Semillas; Argentina
dc.description.fil
Fil: Cravero, Vanina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Martin, Eugenia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Cointry Peix, Enrique Luis. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Producción Vegetal. Cátedra de Mejoramiento Vegetal y Prod.de Semillas; Argentina
dc.journal.title
Australian Journal Of Crop Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.cropj.com/berjeo_8_5_2014_689_696.pdf


Archivos asociados

 

Comunidades y colecciones

Mostrar el registro sencillo del ítem