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dc.contributor.author
Wen, Jian Jun
dc.contributor.author
Zago, María Paola
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dc.contributor.author
Nuñez, Sonia
dc.contributor.author
Gupta, Shivali
dc.contributor.author
Nuñez Burgos, Federico
dc.contributor.author
Garg, Nisha Jain
dc.date.available
2017-12-07T18:13:27Z
dc.date.issued
2012-04
dc.identifier.citation
Wen, Jian Jun; Zago, María Paola; Nuñez, Sonia; Gupta, Shivali; Nuñez Burgos, Federico; et al.; Serum Proteomic Signature of Human Chagasic Patients for the Identification of Novel Potential Protein Biomarkers of Disease; American Society for Biochemistry and Molecular Biology; Molecular & Cellular Proteomics; 11; 4-2012; 435-452
dc.identifier.issn
1535 9476
dc.identifier.uri
http://hdl.handle.net/11336/29986
dc.description.abstract
Chagas disease is initiated upon infection by Trypanosoma cruzi. Among the health consequences is a decline in heart function, and the pathophysiological mechanisms underlying this manifestation are not well understood. To explore the possible mechanisms, we employed IgY LC10 affinity chromatography in conjunction with ProteomeLab PF2D and two-dimensional gel electrophoresis (2D-GE) to resolve the proteome signature of high-abundance and low-abundance serum proteins in chagasic patients. MALDI-TOF MS/MS analysis yielded 80 and 14 differentially expressed proteins associated with cardiomyopathy of chagasic and other etiologies, respectively. The extent of oxidative stress-induced carbonyl modifications of the differentially expressed proteins (n=26) was increased and coupled with a depression of antioxidant proteins. Functional annotation of the top networks developed by Ingenuity Pathway Analysis of proteome database identified dysregulation of inflammation/acute phase response signaling and lipid metabolism relevant to production of prostaglandins and arachidonic acid in chagasic patients. Overlay of the major networks identified prothrombin and plasminogen at a nodal position with connectivity to proteome signature indicative of heart disease (i.e. thrombosis, angiogenesis, vasodilatation of blood vessels or the aorta, increased permeability of blood vessel and endothelial tubes), and inflammatory responses (e.g., platelet aggregation, complement activation, phagocytes activation and migration). The detection of cardiac proteins (myosin light chain 2, myosin heavy chain 11) and increased levels of vinculin and plasminogen provided a comprehensive set of biomarkers of cardiac muscle injury and development of clinical Chagas disease in human patients. These results provide an impetus for biomarker validation in large cohorts of clinically characterized chagasic patients
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Biochemistry and Molecular Biology
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Biomarker
dc.subject
Chagas Disease
dc.subject
Oxidative Stress
dc.subject
T. Cruzi
dc.subject.classification
Otras Ciencias de la Salud
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dc.subject.classification
Ciencias de la Salud
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Serum Proteomic Signature of Human Chagasic Patients for the Identification of Novel Potential Protein Biomarkers of Disease
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-05-29T15:38:06Z
dc.identifier.eissn
1535-9484
dc.journal.volume
11
dc.journal.pagination
435-452
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Rockville
dc.description.fil
Fil: Wen, Jian Jun. University of Texas Medical Branch; Estados Unidos
dc.description.fil
Fil: Zago, María Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
dc.description.fil
Fil: Nuñez, Sonia. Provincia de Salta. Hospital San Bernardo; Argentina
dc.description.fil
Fil: Gupta, Shivali. University of Texas Medical Branch; Estados Unidos
dc.description.fil
Fil: Nuñez Burgos, Federico. Provincia de Salta. Hospital San Bernardo; Argentina
dc.description.fil
Fil: Garg, Nisha Jain. University of Texas Medical Branch; Estados Unidos
dc.journal.title
Molecular & Cellular Proteomics
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dc.relation.isreferencedin
info:eu-repo/semantics/reference/url/http://www.mcponline.org/content/11/8/435
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.mcponline.org/content/11/8/435
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1074/mcp.M112.017640
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412973/
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