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dc.contributor.author
Lodh, Nilanjan  
dc.contributor.author
Caro, Reynaldo  
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Sofer, Shterna  
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Scott, Alan  
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Krolewiecki, Alejandro Javier  
dc.contributor.author
Shiff, Clive  
dc.date.available
2017-12-07T15:20:14Z  
dc.date.issued
2016-07  
dc.identifier.citation
Lodh, Nilanjan; Caro, Reynaldo; Sofer, Shterna; Scott, Alan; Krolewiecki, Alejandro Javier; et al.; Diagnosis of Strongyloides stercoralis: Detection of parasite-derived DNA in urine; Elsevier Science; Acta Tropica; 163; 7-2016; 9-13  
dc.identifier.issn
0001-706X  
dc.identifier.uri
http://hdl.handle.net/11336/29934  
dc.description.abstract
Detecting infections of Strongyloides stercoralis is arduous and has low sensitivity. Clinically this is a major problem because chronic infections may disseminate in the host and lead to a life threatening condition.Epidemiologically, S. stercoralis is often missed in surveys as it is difficult to identify by standard stool examination procedures. We present, for the first time, evidence that the infection can be detected in filtered urine samples collected and processed in the field and subsequently assayed for the presence of parasite DNA. Urine specimens (∼40 mL) were collected from 125 test and control individuals living in rural and peri-urban regions of Northern Argentina. From the same individuals, fresh stool specimens were processed using three different copropological methods. Urine specimens were filtered in the field through a 12.5 cm Whatman No. 3 filter. The filters were dried and packed individually in sealable plastic bags with desiccant and shipped to a laboratory where DNA was recovered from the filter and PCRamplified with primers specific to a dispersed repetitive sequence. Prevalence of S. stercoralis infectionby stool culture and direct examination was 35/125 (28%), In contrast, PCR-based detection of parasitespecific trans-renal DNA in urine indicated that 56/125 (44.8%) carried the parasite. Of the patients that tested positive for urine-based parasite DNA, approximately half also tested positive in their stool specimens. There were 6.4% of cases where parasite larvae were seen in the stool but no DNA was amplified from the urine. As proof of principle, DNA amplification from urine residue reveals significantly more cases of S. stercoralis infection than the current standard stool examination techniques. Additional work is required to establish the relative utility, sensitivity and specificity of urine-based analysis compared to parasitological and nucleic acid detection from stool for clinical and epidemiological detection for S.stercoralis infection.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
Strongyloides Stercoralis  
dc.subject
Diagnostics Test  
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Dna Detection  
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Urine Specimen  
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Gastro-Intestinal Parasite  
dc.subject.classification
Salud Ocupacional  
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Ciencias de la Salud  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Diagnosis of Strongyloides stercoralis: Detection of parasite-derived DNA in urine  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2017-09-01T18:03:50Z  
dc.journal.volume
163  
dc.journal.pagination
9-13  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Ámsterdam  
dc.description.fil
Fil: Lodh, Nilanjan. Marquette University; Estados Unidos  
dc.description.fil
Fil: Caro, Reynaldo. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina  
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Fil: Sofer, Shterna. Johns Hopkins Bloomberg School of Public Health; Estados Unidos  
dc.description.fil
Fil: Scott, Alan. Johns Hopkins Bloomberg School of Public Health; Estados Unidos  
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Fil: Krolewiecki, Alejandro Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina  
dc.description.fil
Fil: Shiff, Clive. Johns Hopkins Bloomberg School of Public Health; Estados Unidos  
dc.journal.title
Acta Tropica  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.actatropica.2016.07.014  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0020751912001701  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5117362/