Artículo
Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis
Großkinsky, Dominik K.; Tafner, Richard; Moreno, Maria Virginia
; Stenglein, Sebastian Alberto
; Garcia, Inés Eugenia; Nelson, Louise M.; Novák, Ondřej; Strnad, Miroslav; Graaff, Eric van der; Roitsch, Thomas
Fecha de publicación:
03/2016
Editorial:
Nature Publishing Group
Revista:
Scientific Reports
ISSN:
2045-2322
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Plant beneficial microbes mediate biocontrol of diseases by interfering with pathogens or via strengthening the host. Although phytohormones, including cytokinins, are known to regulate plant development and physiology as well as plant immunity, their production by microorganisms has not been considered as a biocontrol mechanism. Here we identify the ability of Pseudomonas fluorescens G20-18 to efficiently control P. syringae infection in Arabidopsis, allowing maintenance of tissue integrity and ultimately biomass yield. Microbial cytokinin production was identified as a key determinant for this biocontrol effect on the hemibiotrophic bacterial pathogen. While cytokinindeficientloss-of-function mutants of G20-18 exhibit impaired biocontrol, functional complementation with cytokinin biosynthetic genes restores cytokinin-mediated biocontrol, which is correlated with differential cytokinin levels in planta. Arabidopsis mutant analyses revealed the necessity of functional plant cytokinin perception and salicylic acid-dependent defence signalling for thisbiocontrol mechanism. These results demonstrate microbial cytokinin production as a novel microbebased, hormone-mediated concept of biocontrol. This mechanism provides a basis to potentially develop novel, integrated plant protection strategies combining promotion of growth, a favourable physiological status and activation of fine-tuned direct defence and abiotic stress resilience.
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Articulos(CCT - MAR DEL PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - MAR DEL PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - MAR DEL PLATA
Articulos(INBIOTEC)
Articulos de INSTITUTO DE INV. EN BIODIVERSIDAD Y BIOTECNOLOGIA
Articulos de INSTITUTO DE INV. EN BIODIVERSIDAD Y BIOTECNOLOGIA
Citación
Großkinsky, Dominik K.; Tafner, Richard; Moreno, Maria Virginia; Stenglein, Sebastian Alberto; Garcia, Inés Eugenia; et al.; Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis; Nature Publishing Group; Scientific Reports; 6; 3-2016; 1-11; 23310
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