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dc.contributor.author
Elzagallaai, A.
dc.contributor.author
Rosé, S. D.
dc.contributor.author
Brandan, Nora Cristina
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dc.contributor.author
Trifaro, J. M.
dc.date.available
2017-12-04T18:56:15Z
dc.date.issued
2001-12
dc.identifier.citation
Elzagallaai, A.; Rosé, S. D.; Brandan, Nora Cristina; Trifaro, J. M.; Myristoylated alanine-rich C kinase substrate phosphorylation is involved in thrombin-induced serotonin release from platelets; Wiley; British Journal of Haematology; 112; 3; 12-2001; 593-602
dc.identifier.issn
0007-1048
dc.identifier.uri
http://hdl.handle.net/11336/29595
dc.description.abstract
Stimulation of platelets by thrombin induces protein kinase C (PKC) activation, phosphorylation of pleckstrin, aggregation and serotonin release. Here, we demonstrate that, in human platelets, thrombin stimulation also induced phosphorylation of the myristoylated alanine-rich C Kinase substrate (MARCKS) and serotonine release in intact and digitonin-permeabilized platelets. MARKS is known to bind actin and cross-link actin filaments, and this is inhibited by PKC-evoqued MARKS phosphorylation. MARKS phosphorylation and serotonin release in response to increasing concentration of thrombin have a similar EC 50 and time course and, in permeabilized platelets, peptide MPSD, with an amino sequence corresponding to the phosphorylation site domain of MARKS, bloked both responses. However, pleckstrin and myosin light chain phosphorylations were not modified. Ala-MPSD, in which the four serine residues of MPSD were substituted by alanines was ineffective. The results suggest a role for MARKS in platelet secretion. The fact that pleckstrin phosphorylation has different time course and was not modified in the presence of MPSD when MARCKS phosphorylation and serotonine release were inhibited would suggest either that pleckstrin phosphorylation is unrelated to secretion or that it might only be involved upstream in the events leading to secretion.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Platelets
dc.subject
Thrombin
dc.subject
Markcks
dc.subject
Secretion
dc.subject
Serotonin
dc.subject.classification
Inmunología
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Myristoylated alanine-rich C kinase substrate phosphorylation is involved in thrombin-induced serotonin release from platelets
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-11-03T19:52:37Z
dc.identifier.eissn
1365-2141
dc.journal.volume
112
dc.journal.number
3
dc.journal.pagination
593-602
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Hoboken
dc.description.fil
Fil: Elzagallaai, A.. University of Ottawa; Canadá
dc.description.fil
Fil: Rosé, S. D.. University of Ottawa; Canadá
dc.description.fil
Fil: Brandan, Nora Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. University of Ottawa; Canadá
dc.description.fil
Fil: Trifaro, J. M.. University of Ottawa; Canadá
dc.journal.title
British Journal of Haematology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1046/j.1365-2141.2001.02642.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2141.2001.02642.x
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