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dc.contributor.author
Gardner, Christopher L.
dc.contributor.author
Pagliai, Fernando A.
dc.contributor.author
Pan, Lei
dc.contributor.author
Bojilova, Lora
dc.contributor.author
Torino, Maria Ines
dc.contributor.author
Lorca, Graciela L.
dc.contributor.author
Gonzalez, Claudio F.
dc.date.available
2017-11-29T20:05:28Z
dc.date.issued
2016-10-18
dc.identifier.citation
Gardner, Christopher L.; Pagliai, Fernando A.; Pan, Lei; Bojilova, Lora; Torino, Maria Ines; et al.; Drug repurposing: tolfenamic acid inactivates PrbP, a transcriptional accessory protein in liberibacter asiaticus; Frontiers; Frontiers in Microbiology; 7; 18-10-2016; 1-16;1630-1630
dc.identifier.issn
1664-302X
dc.identifier.uri
http://hdl.handle.net/11336/29307
dc.description.abstract
CLIBASIA_01510, PrbP, is a predicted RNA polymerase binding protein in Liberibacter asiaticus. PrbP was found to regulate expression of a small subset of ribosomal genes through interactions with the β-subunit of the RNA polymerase and a short, specific sequence on the promoter region. Molecular screening assays were performed to identify small molecules that interact with PrbP in vitro. Chemical hits were analyzed for therapeutic efficacy against L. asiaticus via an infected leaf assay, where the transcriptional activity of L. asiaticus was found to decrease significantly after exposure to tolfenamic acid. Similarly, tolfenamic acid was found to inhibit L. asiaticus infection in highly symptomatic citrus seedlings. Our results indicate that PrbP is an important transcriptional regulator for survival of L. asiaticus in planta, and the chemicals identified by molecular screening assays could be used as a therapeutic treatment for huanglongbing disease.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers
dc.rights
info:eu-repo/semantics/openAccess
dc.rights
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Transcriptional Accessory Protein
dc.subject
Liberibacter Asiaticus
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Tolfenamic Acid
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Antimicrobial
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Citrus
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Drug repurposing: tolfenamic acid inactivates PrbP, a transcriptional accessory protein in liberibacter asiaticus
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-13T18:58:42Z
dc.identifier.eissn
1664-302X
dc.journal.volume
7
dc.journal.pagination
1-16;1630-1630
dc.journal.pais
Suiza
dc.journal.ciudad
Lausanne
dc.description.fil
Fil: Gardner, Christopher L.. University of Florida; Estados Unidos
dc.description.fil
Fil: Pagliai, Fernando A.. University of Florida; Estados Unidos
dc.description.fil
Fil: Pan, Lei. University of Florida; Estados Unidos
dc.description.fil
Fil: Bojilova, Lora. University of Florida; Estados Unidos
dc.description.fil
Fil: Torino, Maria Ines. University of Florida; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Lorca, Graciela L.. University of Florida; Estados Unidos
dc.description.fil
Fil: Gonzalez, Claudio F.. University of Florida; Estados Unidos
dc.journal.title
Frontiers in Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fmicb.2016.01630
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2016.01630/full


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