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dc.contributor.author Gardner, Christopher L.
dc.contributor.author Pagliai, Fernando A.
dc.contributor.author Pan, Lei
dc.contributor.author Bojilova, Lora
dc.contributor.author Torino, Maria Ines
dc.contributor.author Lorca, Graciela L.
dc.contributor.author Gonzalez, Claudio F.
dc.date.available 2017-11-29T20:05:28Z
dc.date.issued 2016-10-18
dc.identifier.citation Gardner, Christopher L.; Pagliai, Fernando A.; Pan, Lei; Bojilova, Lora; Torino, Maria Ines; et al.; Drug repurposing: tolfenamic acid inactivates PrbP, a transcriptional accessory protein in liberibacter asiaticus; Frontiers; Frontiers in Microbiology; 7; 18-10-2016; 1-16;1630-1630
dc.identifier.issn 1664-302X
dc.identifier.uri http://hdl.handle.net/11336/29307
dc.description.abstract CLIBASIA_01510, PrbP, is a predicted RNA polymerase binding protein in Liberibacter asiaticus. PrbP was found to regulate expression of a small subset of ribosomal genes through interactions with the β-subunit of the RNA polymerase and a short, specific sequence on the promoter region. Molecular screening assays were performed to identify small molecules that interact with PrbP in vitro. Chemical hits were analyzed for therapeutic efficacy against L. asiaticus via an infected leaf assay, where the transcriptional activity of L. asiaticus was found to decrease significantly after exposure to tolfenamic acid. Similarly, tolfenamic acid was found to inhibit L. asiaticus infection in highly symptomatic citrus seedlings. Our results indicate that PrbP is an important transcriptional regulator for survival of L. asiaticus in planta, and the chemicals identified by molecular screening assays could be used as a therapeutic treatment for huanglongbing disease.
dc.format application/pdf
dc.language.iso eng
dc.publisher Frontiers
dc.rights info:eu-repo/semantics/openAccess
dc.rights Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject TRANSCRIPTIONAL ACCESSORY PROTEIN
dc.subject LIBERIBACTER ASIATICUS
dc.subject TOLFENAMIC ACID
dc.subject ANTIMICROBIAL
dc.subject CITRUS
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Drug repurposing: tolfenamic acid inactivates PrbP, a transcriptional accessory protein in liberibacter asiaticus
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-07-13T18:58:42Z
dc.identifier.eissn 1664-302X
dc.journal.volume 7
dc.journal.pagination 1-16;1630-1630
dc.journal.pais Suiza
dc.journal.ciudad Lausanne
dc.description.fil Fil: Gardner, Christopher L.. University of Florida; Estados Unidos
dc.description.fil Fil: Pagliai, Fernando A.. University of Florida; Estados Unidos
dc.description.fil Fil: Pan, Lei. University of Florida; Estados Unidos
dc.description.fil Fil: Bojilova, Lora. University of Florida; Estados Unidos
dc.description.fil Fil: Torino, Maria Ines. University of Florida; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil Fil: Lorca, Graciela L.. University of Florida; Estados Unidos
dc.description.fil Fil: Gonzalez, Claudio F.. University of Florida; Estados Unidos
dc.journal.title Frontiers in Microbiology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fmicb.2016.01630
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2016.01630/full


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)