Artículo
Expression of Babesia bovis rhoptry-associated protein 1 (RAP1) in Brucella abortus S19
Sabio y Garcia, Julia Veronica
; Farber, Marisa Diana
; Carrica, Mariela del Carmen
; Cravero, Silvio Lorenzo Pedro; Macedo, Gilson C.; Bigi, Fabiana
; Oliveira, Sergio C.; Rossetti, Osvaldo Luis; Campos, Eleonora
Fecha de publicación:
02/2008
Editorial:
Elsevier Science
Revista:
Microbes and Infection
ISSN:
1286-4579
e-ISSN:
1769-714X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Resumen
Brucella abortus strain 19 (live vaccine) induces a strong humoral and cellular immune response and therefore, it is an attractive vector for the delivery of heterologous antigens. The objective of the present study was to express the rhoptry-associated protein (RAP1) of Babesia bovis in B. abortus S19, as a model for heterologous expression of immunostimulatory antigens from veterinary pathogens. A plasmid for the expression of recombinant proteins fused to the aminoterminal of the outer membrane lipoprotein OMP19 was created, pursuing the objective of increasing the immunogenicity of the recombinant antigen being expressed by its association to a lipid moiety. Recombinant strains of B. abortus S19 expressing RAP1 as a fusion protein either with the first amino acids of beta-galactosidase (S19pBB-RAP1) or B. abortus OMP19 (S19pBB19-RAP1) were generated. Plasmid stability and the immunogenicity of the heterologous proteins were analyzed. Mice immunized with S19pBB-RAP1 or S19pBB19-RAP1 developed specific humoral immune response to RAP1, IgG2a being the predominant antibody isotype. Furthermore, a specific cellular immune response to recombinant RAP1 was elicited in vitro by lymphocytes from mice immunized with both strains. Therefore, we concluded that B. abortus S19 expressing RAP1 is immunostimulatory and may provide the basis for combined heterologous vaccines for babesiosis and brucellosis
Palabras clave:
Brucella Abortus
,
Babesia Bovis
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IIBBA)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Sabio y Garcia, Julia Veronica; Farber, Marisa Diana; Carrica, Mariela del Carmen; Cravero, Silvio Lorenzo Pedro; Macedo, Gilson C.; et al.; Expression of Babesia bovis rhoptry-associated protein 1 (RAP1) in Brucella abortus S19; Elsevier Science; Microbes and Infection; 10; 6; 2-2008; 635-641
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