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dc.contributor.author
Tartaglione, M. C.
dc.contributor.author
Ritta, Monica Nora
dc.date.available
2017-11-26T01:04:41Z
dc.date.issued
2004-10
dc.identifier.citation
Tartaglione, M. C.; Ritta, Monica Nora; Prognostic value of spermatological parameters as predictors of in vitro fertility of frozen-thawed bull semen; Elsevier Science Inc; Theriogenology; 62; 7; 10-2004; 1245-1252
dc.identifier.issn
0093-691X
dc.identifier.uri
http://hdl.handle.net/11336/29111
dc.description.abstract
Cryopreservation imposes irreversible damage to sperm membranes, such as swelling and disruption of plasma and acrosome membranes, changes in membrane fluidity, altered influx of calcium, and changes in enzyme activity. Morphological integrity of the sperm plasma membrane has been widely studied using different techniques, including exposure of spermatozoa to hypoosmotic solutions (provides information concerning the biochemical activity of the sperm tail membrane), supravital test using eosin stain (yields information regarding sperm head membrane integrity), and Trypan-blue Giemsa stain (TBG; reveals both sperm plasma membrane and acrosome integrity). The objective of this study was to combine these tests in order to provide information about the integrity of the whole sperm surface, as well as acrosome status, and determine if the results of these tests were associated with sperm in vitro fertilizing ability. Stepwise regression analyses yielded a model in which fertility (maintain variable) was expressed as a combination of the results of different spermatological parameters (independent variables). The results of a test combining supravital eosin staining of samples previously submitted to hypoosmotic swelling test (STHOS) accounted for the greatest proportion of variation in fertilization rates (78%). Inclusion of the results of dual staining with TBG increased the proportion of variation in fertility rate that could be accounted for to 82%. Therefore, sperm plasma membrane integrity and function, and acrosome integrity can be considered important variables for normal sperm function and STHOST and TBG could be used for the prognosis of the potential fertility of bovine semen samples used for IVF or AI.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Sperm
dc.subject
Bovine
dc.subject
Parameters
dc.subject
Fertility
dc.subject
Semen Preservation
dc.subject.classification
Otras Biotecnología Agropecuaria
dc.subject.classification
Biotecnología Agropecuaria
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
Prognostic value of spermatological parameters as predictors of in vitro fertility of frozen-thawed bull semen
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-11-16T15:12:42Z
dc.identifier.eissn
1879-3231
dc.journal.volume
62
dc.journal.number
7
dc.journal.pagination
1245-1252
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Tartaglione, M. C.. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; Argentina
dc.description.fil
Fil: Ritta, Monica Nora. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.journal.title
Theriogenology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.theriojournal.com/article/S0093-691X(04)00085-8/abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.theriogenology.2004.01.012
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/15325551
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