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dc.contributor.author
Abeijon Mukdsi, Maria Claudia
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dc.contributor.author
Falentin, Hélène
dc.contributor.author
Maillard, Marie Bernadette
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dc.contributor.author
Chuat, Victoria
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dc.contributor.author
Medina, Roxana Beatriz
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dc.contributor.author
Parayre, Sandrine
dc.contributor.author
Thierry, Anne
dc.date.available
2015-11-24T17:00:49Z
dc.date.issued
2014-01
dc.identifier.citation
Abeijon Mukdsi, Maria Claudia; Falentin, Hélène ; Maillard, Marie Bernadette ; Chuat, Victoria; Medina, Roxana Beatriz; et al.; The secreted esterase of propionibacterium freudenreichii has a major role in cheese lipolysis; American Society for Microbiology; Applied And Environmental Microbiology; 80; 2; 1-2014; 751-756
dc.identifier.issn
0099-2240
dc.identifier.uri
http://hdl.handle.net/11336/2908
dc.description.abstract
Free fatty acids are important flavor compounds in cheese. Propionibacterium freudenreichii is the main agent of their release through lipolysis in Swiss cheese. Our aim was to identify the esterase(s) involved in lipolysis by P. freudenreichii. We targeted two previously identified esterases: one secreted esterase, PF#279, and one putative cell wall-anchored esterase, PF#774. To evaluate
their role in lipolysis, we constructed overexpression and knockout mutants of P. freudenreichii CIRM-BIA1T for each corresponding gene. The sequences of both genes were also compared in 21 wild-type strains. All strains were assessed for their lipolytic activity on milk fat. The lipolytic activity observed matched data previously reported in cheese, thus validating the relevance
of the method used. The mutants overexpressing PF#279 or PF#774 released four times more fatty acids than the wild-type strain, demonstrating that both enzymes are lipolytic esterases. However, inactivation of the pf279 gene induced a 75% reduction in the lipolytic activity compared to that of the wild-type strain, whereas inactivation of the pf774 gene did not modify the
phenotype. Two of the 21 wild-type strains tested did not display any detectable lipolytic activity. Interestingly, these two strains exhibited the same single-nucleotide deletion at the beginning of the pf279 gene sequence, leading to a premature stop codon, whereas they harbored a pf774 gene highly similar to that of the other strains. Taken together, these results clearly demonstrate that PF#279 is the main lipolytic esterase in P. freudenreichii and a key agent of Swiss cheese lipolysis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Microbiology
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Esterase
dc.subject
Propionibacterium Freudenreichii
dc.subject
Cheese Lipolysis
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
The secreted esterase of propionibacterium freudenreichii has a major role in cheese lipolysis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.identifier.eissn
1098-5336
dc.journal.volume
80
dc.journal.number
2
dc.journal.pagination
751-756
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Washington
dc.description.fil
Fil: Abeijon Mukdsi, Maria Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
dc.description.fil
Fil: Falentin, Hélène. INRA. Science et Technologie du Lait et de l’OEuf; Francia. Agrocampus Ouest. Science et Technologie du Lait et de l’OEuf; Francia
dc.description.fil
Fil: Maillard, Marie Bernadette. INRA. Science et Technologie du Lait et de l’OEuf; Francia. Agrocampus Ouest. Science et Technologie du Lait et de l’OEuf; Francia
dc.description.fil
Fil: Chuat, Victoria. INRA. Science et Technologie du Lait et de l’OEuf; Francia. Agrocampus Ouest. Science et Technologie du Lait et de l’OEuf; Francia
dc.description.fil
Fil: Medina, Roxana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
dc.description.fil
Fil: Parayre, Sandrine. INRA. Science et Technologie du Lait et de l’OEuf; Francia. Agrocampus Ouest. Science et Technologie du Lait et de l’OEuf; Francia
dc.description.fil
Fil: Thierry, Anne. INRA. Science et Technologie du Lait et de l’OEuf; Francia. Agrocampus Ouest. Science et Technologie du Lait et de l’OEuf; Francia
dc.journal.title
Applied And Environmental Microbiology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/http://www.pubmedcentral.nih.gov/tocrender.fcgi?journal=83
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/doi:10.1128/AEM.03640-13
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