Artículo
Polymerase chain reaction-based assay for the detection and identification of sand fly gregarines in Lutzomyia longipalpis, vector of visceral leishmaniasis
Caligiuri, Lorena Gisel; Acardi, Soraya Alejandra
; Santini, Maria Soledad
; Salomón, Oscar Daniel
; Mccarthy, Cristina Beryl
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Fecha de publicación:
06/2014
Editorial:
Wiley
Revista:
Journal of Vector Ecology
ISSN:
1081-1710
e-ISSN:
1948-713
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Gregarines that parasitise phlebotomine sand flies belong to the genus Psychodiella and, even though they are highly host-specific, only five species have been described to date. Their most outstanding features include the unique localisation of the oocysts in the accessory glands of the female host, which ensures contamination of the egg surface during oviposition, and the fact that they naturally parasitise the vectors of Leishmania, causal agent of leishmaniasis. The type species, Ps. chagasi, was first described in Lutzomyia longipalpis, vector of visceral leishmaniasis (VL), from Brazil. We recently reported Ps. chagasi sequences in Lu. longipalpis from Posadas (Misiones, Argentina), an endemic VL location where this gregarine had not been previously recorded. In order to analyse the incidence of Ps. chagasi infections in Lu. longipalpis from this location, the aim of this study was to develop a diagnostic assay for sand fly gregarine parasites in Lu. longipalpis. For this, we designed primers using the Ps. chagasi sequences we previously identified and performed an in vitro validation by PCR amplification of the original sand fly samples. Their specificity and sensitivity as diagnostic primers were subsequently confirmed by PCR reactions using total DNA extracted from naturally infected Lu. longipalpis from the same location (Posadas, Argentina).
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Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(CCT - NORDESTE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NORDESTE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NORDESTE
Citación
Caligiuri, Lorena Gisel; Acardi, Soraya Alejandra; Santini, Maria Soledad; Salomón, Oscar Daniel; Mccarthy, Cristina Beryl; Polymerase chain reaction-based assay for the detection and identification of sand fly gregarines in Lutzomyia longipalpis, vector of visceral leishmaniasis; Wiley; Journal of Vector Ecology; 39; 1; 6-2014; 83-93
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