Mostrar el registro sencillo del ítem

dc.contributor.author
Martos, María Alicia
dc.contributor.author
Butiuk, Ana Paula
dc.contributor.author
Rojas, Natalia Lorena
dc.contributor.author
Hours, Roque Alberto
dc.date.available
2017-11-24T19:21:20Z
dc.date.issued
2014-07
dc.identifier.citation
Martos, María Alicia; Butiuk, Ana Paula; Rojas, Natalia Lorena; Hours, Roque Alberto; Purification and Characterization of a Polygalacturonase Produced by Wickerhamomyces anomalus; Inst Tecnologia Parana; Brazilian Archives Of Biology And Technology; 57; 4; 7-2014; 587-594
dc.identifier.issn
1516-8913
dc.identifier.uri
http://hdl.handle.net/11336/29058
dc.description.abstract
The aim of this work was to study the purification and physicochemical properties of an endo-polygalacturonase(PG) produced by Wickerhamomyces anomalus isolated from the citrus fruit peels. The enzyme was purified to homogeneity from the culture filtrate of W. anomalus grown on the yeast nitrogen base medium with glucose as carbon and energy source and citrus pectin as inductor. After anion-exchange chromatography and gel filtration chromatography, PG activity was eluted as a single peak, yielding 21% of the original activity. After dialysis and cation-exchange chromatography, only one fraction with PG activity was obtained, recovering 56% of initial enzyme activity and 1.3-fold increase in specific activity. The molecular weight of the enzyme was estimated as 43 kDa by the SDS-PAGE. The enzyme exhibited maximal activity at pH 4.2 and was stable over a pH range from 3.5 to 6.0 and up to 49ºC for 10 h. The Vmax and Km values with polygalacturonic acid as substrate were 0.26 mmol/L.min and 0.173 mg/mL, respectively. Cations such as Cu+2, Fe+3, Mg+2, Mn+2 and Zn+2 did not show any significant effect on PG activity but K+ and Ca+2 reduced it. The purified PG was able to macerate cassava tissues.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Inst Tecnologia Parana
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Wickerhamomyces Anomalus
dc.subject
Polygalacturonase
dc.subject
Purification
dc.subject
Characterization
dc.subject.classification
Biotecnología Industrial
dc.subject.classification
Biotecnología Industrial
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
Purification and Characterization of a Polygalacturonase Produced by Wickerhamomyces anomalus
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-11-16T14:53:01Z
dc.identifier.eissn
1678-4324
dc.journal.volume
57
dc.journal.number
4
dc.journal.pagination
587-594
dc.journal.pais
Brasil
dc.journal.ciudad
Curitiba
dc.description.fil
Fil: Martos, María Alicia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
dc.description.fil
Fil: Butiuk, Ana Paula. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Rojas, Natalia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
dc.description.fil
Fil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
dc.journal.title
Brazilian Archives Of Biology And Technology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1590/S1516-8913201402214
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/9jwt99


Archivos asociados

 

Comunidades y colecciones

Mostrar el registro sencillo del ítem