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dc.contributor.author
Labombarda, Maria Florencia
dc.contributor.author
Pianos, A.
dc.contributor.author
Liere, P.
dc.contributor.author
Eychenne, B.
dc.contributor.author
Gonzalez, Susana Laura
dc.contributor.author
Cambourg, A.
dc.contributor.author
de Nicola, Alejandro Federico
dc.contributor.author
Schumacher, Michael
dc.contributor.author
Guennoun, Rachida
dc.date.available
2017-11-23T17:04:46Z
dc.date.issued
2006-04
dc.identifier.citation
Labombarda, Maria Florencia; Pianos, A.; Liere, P.; Eychenne, B. ; Gonzalez, Susana Laura; et al.; Injury elicited increase in spinal cord neurosteroid content analysed by gas chromatography mass espectrometry; Endocrine Society; Endocrinology; 147; 4; 4-2006; 1847-1859
dc.identifier.issn
0013-7227
dc.identifier.uri
http://hdl.handle.net/11336/28879
dc.description.abstract
The effects of spinal cord injury (SCI), combined with castration and adrenalectomy, and of progesterone (PROG) treatment on neurosteroid levels and steroidogenic enzyme expression were investigated in the adult male rat spinal cord (SC). Steroid levels were quantified by gas chromatography/mass spectrometry in SC and plasma, and mRNAs of enzymes by quantitative real-time RT-PCR. The levels of pregnenolone (PREG), PROG, 5alpha-dihydroprogesterone, 3alpha,5alpha-tetrahydroprogesterone increased in SC 75 h after transection without significant increase in the plasma. After combined adrenalectomy and gonadectomy, significant levels of PREG and PROG remained in the SC, suggesting their local biosynthesis. In the SC of adrenalectomized and gonadectomized rats, there was an increase of PREG 24 h after SCI, followed at 75 h by a concomitant increase in its direct metabolite, PROG. These observations are consistent with a sequential increase of PREG biosynthesis and its conversion to PROG within the SC in response to injury. However, no significant change in P450-side chain cleavage and 3beta-hydroxysteroid dehydrogenase/Delta5-Delta4 isomerase mRNA levels was observed after SCI. Systemic PROG treatment after SCI, resulted in a very large increase in PROG, 5alpha-dihydroprogesterone, and 3alpha,5alpha-tetrahydroprogesterone in both plasma and SC. Furthermore, high levels of 3beta,5alpha-tetrahydroprogesterone were detected in SC, whereas their plasma levels remained barely detectable. Because the ratio of reduced metabolites to PROG was 65-times higher in SC than in the plasma, it appears likely that reduced metabolites mainly originated from local biosynthesis. Our results strongly suggest an important role for locally biosynthesized neurosteroids in the response of the SC to injury.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Endocrine Society
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Neurosteroids
dc.subject
Spinal Cord Injury
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Pregnanolone
dc.subject
Progesterone
dc.subject.classification
Neurociencias
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Injury elicited increase in spinal cord neurosteroid content analysed by gas chromatography mass espectrometry
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-11-02T20:11:29Z
dc.identifier.eissn
1945-7170
dc.journal.volume
147
dc.journal.number
4
dc.journal.pagination
1847-1859
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Labombarda, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Inserm; Francia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
dc.description.fil
Fil: Pianos, A.. Inserm; Francia
dc.description.fil
Fil: Liere, P.. Inserm; Francia
dc.description.fil
Fil: Eychenne, B.. Inserm; Francia
dc.description.fil
Fil: Gonzalez, Susana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Inserm; Francia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
dc.description.fil
Fil: Cambourg, A.. Inserm; Francia
dc.description.fil
Fil: de Nicola, Alejandro Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Inserm; Francia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
dc.description.fil
Fil: Schumacher, Michael. Inserm; Francia
dc.description.fil
Fil: Guennoun, Rachida. Inserm; Francia
dc.journal.title
Endocrinology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/endo/article/147/4/1847/2501046
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1210/en.2005-0955
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/16396987


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  • Articulos(IBYME) [1100]
    Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)

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