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Artículo

Enhanced seed germination of Ilex dumosa R. (Aquifoliaceae) through in vitro culture of cut pyrenes

Dolce, Natalia RaquelIcon ; Mroginski, Luis AmadoIcon ; Rey, Hebe Yolanda
Fecha de publicación: 02/2011
Editorial: Amer Soc Horticultural Science
Revista: Hortscience
ISSN: 0018-5345
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Biotecnología Agropecuaria

Resumen

An in vitro culture protocol was developed that increased the germination percentage and decreased the lag time to germination for Ilex dumosa R. pyrenes as a tool for replacing the laborious task of embryo rescue technique. This method involves transversely cutting surface-sterilized pyrenes with a scalpel blade, then placing the micropylar one-third end with the rudimentary embryo (0.25 mm long) on solidified (agar 0.65%) quarter-strength salts and vitamins of Murashige and Skoog, 1962 medium with 3% sucrose, and incubating in a growth room at 27 ± 2 8C with a 14-h photoperiod (116 mmolm?2 s?1). Most of the cut pyrenes (greater than 50%) germinated within the first month after inoculation and achieved maximum germination (70%) in 2 months compared with whole pyrenes, which began to germinate 3 months after sowing and required more than 8 months for maximum germination (37%). Moreover, the germination percentage of cut pyrenes was significantly higher than the germination of isolated embryos (34%). Thus, the cut pyrenes culture is a simpler and more effective technique than embryo rescue. Easily, on average, a trained operator is able to culture ~1000 cut pyrenes per day instead of ~100 isolated embryos.
Palabras clave: Embryo Rescue , Seed Dormancy , Light , Cold Storage , Holly
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/2848
URL: http://hortsci.ashspublications.org/content/46/2/278.full.pdf+html
Colecciones
Articulos(CCT - NORDESTE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NORDESTE
Articulos(IBONE)
Articulos de INST.DE BOTANICA DEL NORDESTE (I)
Citación
Dolce, Natalia Raquel; Mroginski, Luis Amado; Rey, Hebe Yolanda; Enhanced seed germination of Ilex dumosa R. (Aquifoliaceae) through in vitro culture of cut pyrenes; Amer Soc Horticultural Science; Hortscience; 46; 2; 2-2011; 278–281
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