Artículo
p8/nupr1 regulates DNA-repair activity after double-strand gamma irradiation-induced DNA damage
Gironella, Meritxell; Malicet, Cedric; Cano, Carla; Sandi, Maria Jose; Hamidi, Tewfic; Neme Tauil, Ricardo Martin
; Baston, Mariela; Valacco, Maria Pia
; Moreno, Silvia Margarita
; Lopez, Frederic; Neira, Jose Luis; Dagorn, Juan Charles; Iovanna, Juan Lucio
Fecha de publicación:
12/2009
Editorial:
Wiley
Revista:
Journal of Cellular Physiology
ISSN:
0021-9541
e-ISSN:
1097-4652
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The stress protein p8 is a small, highly basic, unfolded, and multifunctional protein. We have previously shown that most of its functions are exerted through interactions with other proteins, whose activities are thereby enhanced or repressed. In this work we describe another example of such mechanism, by which p8 binds and negatively regulates MSL1, a histone acetyl transferase (HAT)-associated protein, which in turn binds the DNA-damage-associated 53BP1 protein to facilitate DNA repair following DNA gamma-irradiation. Contrary to the HAT-associated activity, MSL1-dependent DNA-repair activity is almost completely dependent on 53BP1 expression. The picture that has emerged from our findings is that 53BP1 could be a scaffold that gets the HAT MSL1-dependent DNA-repair activity to the sites of DNA damage. Finally, we also found that, although p8 expression is transiently activated after gamma-irradiation, it is eventually submitted to sustained down-regulation, presumably to allow development of MSL1-associated DNA-repair activity. We conclude that interaction of MSL1 with 53BP1 brings MSL1-dependent HAT activity to the vicinity of damaged DNA. MSL1-dependent HAT activity, which is negatively regulated by the stress protein p8, induces chromatin remodeling and relaxation allowing access to DNA of the repair machinery
Palabras clave:
P8
,
Msl1
,
Dna Repair
,
Hat
,
Yeast Two-Hybrid Screening
,
Tp53bp1
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Colecciones
Articulos(IIBBA)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Citación
Gironella, Meritxell; Malicet, Cedric; Cano, Carla; Sandi, Maria Jose; Hamidi, Tewfic; et al.; p8/nupr1 regulates DNA-repair activity after double-strand gamma irradiation-induced DNA damage; Wiley; Journal of Cellular Physiology; 221; 3; 12-2009; 594-602
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