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dc.contributor.author
Diniz Luerce, Tessália
dc.contributor.author
Santiago Pacheco Azevedo, Marcela
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Leblanc, Jean Guy Joseph
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Azevedo, Vasco
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Miyoshi, Anderson
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Santos Pontes, Daniela
dc.date.available
2015-11-17T16:13:37Z
dc.date.issued
2014-11
dc.identifier.citation
Diniz Luerce, Tessália; Santiago Pacheco Azevedo, Marcela; Leblanc, Jean Guy Joseph; Azevedo, Vasco; Miyoshi, Anderson; et al.; Recombinant lactococcus lactis fails to secrete bovine chymosine; Taylor & Francis; Bioengineered; 5; 6; 11-2014; 363-370
dc.identifier.issn
2165-5979
dc.identifier.uri
http://hdl.handle.net/11336/2835
dc.description.abstract
Bovine chymosin is an important milk-clotting agent used in the manufacturing of cheeses. Currently, the production of recombinant proteins by genetically modified organisms is widespread, leading to greatly reduced costs. Lactococcus (L.) lactis, the model lactic acid bacterium, was considered a good candidate for heterologous chymosin production for the following reasons: (1) it is considered to be a GRAS (generally regarded as safe) microorganism, (2) only one protease is present on its surface, (3) it can secrete proteins of different sizes, and (4) it allows for the direct production of protein in fermented food products. Thus, three genetically modified L. lactis strains were constructed to produce and target the three different forms of bovine chymosin, prochymosin B, chymosin A and chymosin B to the extracellular medium. Although all three proteins were stably produced in L. lactis, none of the forms were detected in the extracellular medium or showed clotting activity in milk. Our hypothesis is that this secretion deficiency and lack of clotting activity can be explained by the recombinant protein being attached to the cell envelope. Thus, the development of other strategies is necessary to achieve both production and targeting of chymosin in L. lactis, which could facilitate the downstream processing and recovery of this industrially important protein.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Taylor & Francis
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
BOVINE CHYMOSIN
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BOVINE PROCHYMOSIN
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HETEROLOGOUS EXPRESSION
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LACTOCOCCUS LACTIS
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Otras Ingenierías y Tecnologías
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Otras Ingenierías y Tecnologías
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Recombinant lactococcus lactis fails to secrete bovine chymosine
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.identifier.eissn
2165-5987
dc.journal.volume
5
dc.journal.number
6
dc.journal.pagination
363-370
dc.journal.pais
Estados Unidos
dc.conicet.avisoEditorial
This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/ by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
dc.description.fil
Fil: Diniz Luerce, Tessália. Universidade Federal de Minas Gerais; Brasil
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Fil: Santiago Pacheco Azevedo, Marcela. Universidade Federal de Minas Gerais; Brasil
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Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
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Fil: Azevedo, Vasco. Universidade Federal de Minas Gerais; Brasil
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Fil: Miyoshi, Anderson. Universidade Federal de Minas Gerais; Brasil
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Fil: Santos Pontes, Daniela. Universidade Estadual da Paraiba; Brasil
dc.journal.title
Bioengineered
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4161/bioe.36327
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