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dc.contributor.author Suárez, Nadia Elina
dc.contributor.author Bonacina, Julieta
dc.contributor.author Hebert, Elvira Maria
dc.contributor.author Saavedra, Maria Lucila
dc.date.available 2017-11-15T18:16:06Z
dc.date.issued 2015-10-18
dc.identifier.citation Suárez, Nadia Elina; Bonacina, Julieta; Hebert, Elvira Maria; Saavedra, Maria Lucila; Genome mining and transcriptional analysis of bacteriocin genes in enterococcus faecium CRL1879; OMICS International; Journal of Data Mining in Genomics & Proteomics; 6; 3; 18-10-2015; 1-8;1000180-1000180
dc.identifier.issn 2153-0602
dc.identifier.uri http://hdl.handle.net/11336/28258
dc.description.abstract Among 151 bacterial isolates from nine artisanal cheeses, Enterococcus faecium CRL 1879 showed antibacterialactivity against the food-borne pathogen Listeria monocytogenes. The isolate produced a proteinase K-sensitivecompound in the cell free supernatant. Genome analysis demonstrated the presence of enterocin A, enterocin B,enterocin P, enterocin SE-K4-like and enterocin X biosynthetic gene clusters. Nucleotide sequences encoding for aputative two-component bacteriocin were detected using bioinformatics tools, here named enterocin CRL1879αβ.A transcriptional analysis of all bacteriocin genes by quantitative real time PCR analysis (qRT-PCR) revealed thetranscription of each enterocin gene at different levels. Finally, analysis of bacteriocin genes distribution in 251 E.faecium bioprojects was performed and compared to those identify in E. faecium CRL1879. The discriminative analysisdemonstrated that bacteriocin genes are widely distributed among Enterococcus, independently of the origin of thestrain.The results presented in this paper represent a unique finding since this is the first demonstration of an E. faeciumstrain isolated from an artisanal cheese with the complete genetic machinery to produce six classes II and one class III bacteriocins.
dc.format application/pdf
dc.language.iso eng
dc.publisher OMICS International
dc.rights info:eu-repo/semantics/openAccess
dc.rights Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject BACTERIOCINS
dc.subject ENTEROCOCCUS FAECIUM
dc.subject GENOMES
dc.subject TRANSCRIPTIONAL ANALYSIS
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Genome mining and transcriptional analysis of bacteriocin genes in enterococcus faecium CRL1879
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-07-17T13:47:02Z
dc.journal.volume 6
dc.journal.number 3
dc.journal.pagination 1-8;1000180-1000180
dc.journal.pais Estados Unidos
dc.journal.ciudad Washington DC
dc.description.fil Fil: Suárez, Nadia Elina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil Fil: Bonacina, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil Fil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil Fil: Saavedra, Maria Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.journal.title Journal of Data Mining in Genomics & Proteomics
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4172/2153-0602.1000180
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://www.omicsonline.org/open-access/genome-mining-and-transcriptional-analysis-of-bacteriocin-genes-inenterococcus-faecium-crl1879-2153-0602-1000180.php?aid=62144


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)