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dc.contributor.author Moredo, Fabiana Alicia
dc.contributor.author Piñeyro, Pablo
dc.contributor.author Márquez, Gabriela
dc.contributor.author Sanz, Marcelo Eduardo
dc.contributor.author Colello, Rocío
dc.contributor.author Etcheverría, Analía Inés
dc.contributor.author Padola, Nora Lía
dc.contributor.author Quiroga, María Alejandra
dc.contributor.author Perfumo, Carlos Juan
dc.contributor.author Galli, Lucía
dc.contributor.author Leotta, Gerardo Anibal
dc.date.available 2017-11-10T20:23:14Z
dc.date.issued 2015-08
dc.identifier.citation Moredo, Fabiana Alicia; Piñeyro, Pablo; Márquez, Gabriela; Sanz, Marcelo Eduardo; Colello, Rocío; et al.; Enterotoxigenic escherichia coli subclinical infection in pigs: bacteriological and genotypic characterization and antimicrobial resistance profiles; Mary Ann Liebert Inc; Foodborne Pathogens And Disease; 12; 8; 8-2015; 704-711
dc.identifier.issn 1535-3141
dc.identifier.uri http://hdl.handle.net/11336/27955
dc.description.abstract Enterotoxigenic Escherichia coli (ETEC) is the major pathogen responsible for neonatal diarrhea, postweaning diarrhea, and edema disease in pigs. Although it can be harmless, ETEC is also present in the intestines of other animal species and humans, causing occasional diarrhea outbreaks. The evaluation of this pathogen’s presence in food sources is becoming an increasingly important issue in human health. In order to determine the prevalence of ETEC in nondiarrheic pigs, 990 animals from 11 pig farms were sampled. Using end-time polymerase chain reaction (PCR), eltA, estI genes, or both, were detected in 150 (15.2%) animals. From the positive samples, 40 (26.6%) ETEC strains were isolated, showing 19 antibiotic-resistance patterns; 52.5% of these strains had multiple antibiotic resistances, and 17.5% carried the intI2 gene. The most prevalent genotypes were rfbO157/estII/aidA (32.5%) and estI/estII (25.0%). The estII gene was identified most frequently (97.5%), followed by estI (37.5%), astA (20.0%), and eltA (12.5%). The genes coding the fimbriae F5, F6, and F18 were detected in three single isolates. The aidA gene was detected in 20 ETEC strains associated with the estII gene. Among the isolated ETEC strains, stx2e/estI, stx2e/estI/estII, and stx2e/estI/estII/intI2 genotypes were identified. The ETEC belonged to 12 different serogroups; 37.5% of them belonged to serotype O157:H19. Isolates were grouped by enterobacterial repetitive intergenic consensus–PCR into 5 clusters with 100.0% similarity. In this study, we demonstrated that numerous ETEC genotypes cohabit and circulate in swine populations without clinical manifestation of neonatal diarrhea, postweaning diarrhea, or edema disease in different production stages. The information generated is important not only for diagnostic and epidemiological purposes, but also for understanding the dynamics and ecology of ETEC in pigs in different production stages that can be potentially transmitted to humans from food animals.
dc.format application/pdf
dc.language.iso eng
dc.publisher Mary Ann Liebert Inc
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject ETEC
dc.subject non-diarrheic pigs
dc.subject serotypes
dc.subject antimicrobial resistant
dc.subject.classification Otras Ciencias Veterinarias
dc.subject.classification Ciencias Veterinarias
dc.subject.classification CIENCIAS AGRÍCOLAS
dc.title Enterotoxigenic escherichia coli subclinical infection in pigs: bacteriological and genotypic characterization and antimicrobial resistance profiles
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-01-17T19:20:36Z
dc.identifier.eissn 1556-7125
dc.journal.volume 12
dc.journal.number 8
dc.journal.pagination 704-711
dc.journal.pais Estados Unidos
dc.journal.ciudad Nueva York
dc.description.fil Fil: Moredo, Fabiana Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
dc.description.fil Fil: Piñeyro, Pablo. University of Iowa; Estados Unidos
dc.description.fil Fil: Márquez, Gabriela. Virginia Tech; Estados Unidos
dc.description.fil Fil: Sanz, Marcelo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
dc.description.fil Fil: Colello, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
dc.description.fil Fil: Etcheverría, Analía Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
dc.description.fil Fil: Padola, Nora Lía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
dc.description.fil Fil: Quiroga, María Alejandra. Universidad Nacional de la Plata; Argentina
dc.description.fil Fil: Perfumo, Carlos Juan. Universidad Nacional de la Plata; Argentina
dc.description.fil Fil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
dc.description.fil Fil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
dc.journal.title Foodborne Pathogens And Disease
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/26217917
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1089/fpd.2015.1959


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)