Hypoxia induced by disruption of FGF signalling impairs moulting via insulin and neurohormonal regulation in Rhodnius prolixus

Abstract

The tracheal system of insects undergoes dynamic changes during growth to meet increasing oxygen demands, which are crucial for processes such as moulting. Studies in model organisms, such as Drosophila melanogaster, have identified key genetic regulators of tracheal development, including components of the FGF signalling pathway – branchless (bnl) and breathless (btl). The present study centres on the role of FGFR and bnl in Rhodnius prolixus in tracheogenesis and moulting regulation. Here we identified R. prolixus FGF signalling pathway orthologues implicated in tracheogenesis, providing insight into their conserved function in regulating tracheal development in this species. Insects with knockdown of Rp-bnl and Rp-FGFR showed disruptions in three major biological processes: tracheogenesis, moulting and metabolic homeostasis. Gene expression analysis revealed that downregulation of Rp-bnl and Rp-FGFR expression induced tissue hypoxia, altered the expression of critical moulting regulators and modified metabolic pathways, by affecting the expression of central metabolic factors involved in carbohydrate and lipid metabolism, such as Insulin-like peptide and Adipokinetic hormone. Our work provides the first characterisation of the Rp-Bnl/FGFR pathway in R. prolixus. It shows its central role in coordinating tracheogenesis, developmental timing and the regulation of metabolic pathways, providing new insights into the molecular mechanisms behind these interconnected developmental and physiological processes under hypoxic stress.