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dc.contributor.author
Viola, Ivana Lorena  
dc.contributor.author
Gonzalez, Daniel Hector  
dc.contributor.other
Yuan, Ling  
dc.contributor.other
Perry, Sharyn E.  
dc.date.available
2025-11-06T14:53:17Z  
dc.date.issued
2011  
dc.identifier.citation
Viola, Ivana Lorena; Gonzalez, Daniel Hector; Footprinting and Missing Nucleoside Analysis of Transcription Factor–DNA Complexes; Humana Press; 2011; 259-275  
dc.identifier.isbn
978-1-61779-153-6  
dc.identifier.uri
http://hdl.handle.net/11336/275081  
dc.description.abstract
In the following chapter we describe methods and protocols to analyze the interaction of proteins with DNA using footprinting and related techniques based on the modification of DNA with either hydroxyl radicals or methylating agents. Footprinting, based on the protection from chemical modification of DNA through the specific binding of a protein, gives information about the nucleotides that are in close contact with the protein upon binding. The derived missing nucleoside and interference techniques identify nucleotides that are energetically important for protein binding. These methods are highly valuable to study in detail the interaction of a transcription factor with nucleotides on both strands of its target DNA sequence.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Humana Press  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
FOOTPRINTING  
dc.subject
MISSING NUCLEOSIDE  
dc.subject
PROTEINDNA INTERACTION  
dc.subject
TRANSCRIPTION FACTOR  
dc.subject
METHYLATION INTERFERENCE -  
dc.subject
HYDROXYL RADICAL  
dc.subject.classification
Biología Celular, Microbiología  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Footprinting and Missing Nucleoside Analysis of Transcription Factor–DNA Complexes  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.type
info:eu-repo/semantics/bookPart  
dc.type
info:ar-repo/semantics/parte de libro  
dc.date.updated
2020-12-22T16:42:22Z  
dc.journal.pagination
259-275  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Nueva York  
dc.description.fil
Fil: Viola, Ivana Lorena. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina  
dc.description.fil
Fil: Gonzalez, Daniel Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Biología Celular y Molecular; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007/978-1-61779-154-3_15  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/978-1-61779-154-3_15  
dc.conicet.paginas
347  
dc.source.titulo
Plant Transcription Factors: Methods and Protocols  

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