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Artículo

A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop

Palm, Thomas; Hemmer, Kathrin; Winter, Julia; Fricke, Inga B.; Tarbashevich, Katsiaryna; Sadeghi Shakib, Fereshteh; Rudolph, Ina-Maria; Hillje, Anna Lena; de Luca, PaolaIcon ; Bahnassawy, Lamia’a; Madel, Rabea; Viel, Thomas; de Siervi, AdrianaIcon ; Jacobs, Andreas H.; Diederichs, Sven; Schwamborn, Jens C.
Fecha de publicación: 08/02/2013
Editorial: Oxford University Press
Revista: Nucleic Acids Research
ISSN: 0305-1048
e-ISSN: 1362-4962
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Celular, Microbiología

Resumen

Stem cell fate decisions are controlled by a molecular network in which transcription factors and miRNAs are of key importance. To systemically investigate their impact on neural stem cell (NSC) maintenance and neuronal commitment, we performed a high-throughput mRNA and miRNA profiling and isolated functional interaction networks of involved mechanisms. Thereby, we identified an E2F1–miRNA feedback loop as important regulator of NSC fate decisions. Although E2F1 supports NSC proliferation and represses transcription of miRNAs from the miR-17∼92 and miR-106a∼363 clusters, these miRNAs are transiently up-regulated at early stages of neuronal differentiation. In these early committed cells, increased miRNAs expression levels directly repress E2F1 mRNA levels and inhibit cellular proliferation. In mice, we demonstrated that these miRNAs are expressed in the neurogenic areas and that E2F1 inhibition represses NSC proliferation. The here presented data suggest a novel interaction mechanism between E2F1 and miR-17∼92 / miR-106a∼363 miRNAs in controlling NSC proliferation and neuronal differentiation.
Palabras clave: E2f-1 , Neural Stem Cell
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial 2.5 Unported (CC BY-NC 2.5)
Identificadores
URI: http://hdl.handle.net/11336/2750
URL: http://nar.oxfordjournals.org/content/41/6/3699.long
URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3616707/
DOI: http://dx.doi.org/10.1093%2Fnar%2Fgkt070
Colecciones
Articulos(IQUIBICEN)
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Citación
Palm, Thomas; Hemmer, Kathrin; Winter, Julia; Fricke, Inga B.; Tarbashevich, Katsiaryna; et al.; A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop; Oxford University Press; Nucleic Acids Research; 41; 6; 8-2-2013; 3699-3712
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