Biotechnological Potential of Trichoderma koningiopsis LBM116 as a Natural Source of Micolytic Enzymes for Biocontrol

Abstract

The rapid growth of the global population and the need for sustainable agricultural practices have driven interest in natural biocontrol agents. This study explores the biotechnological potential of Trichoderma koningiopsis LBM116 as a natural source of hydrolytic enzymes, including chitinases, β−1,3-glucanases, and proteases, with promising applications in biological control. The enzymatic formulation derived from this strain exhibited significant inhibitory effects In Vitro on the phytopathogen Alternaria sp., reducing its growth by 64% compared to control treatments. Biochemical characterization of enzymes involved in the biological control of Alternaria sp. was performed. This revealed optimal activity for chitinases and proteases at 45°C (pH 4.8 and 7.4, respectively) and for β−1,3-glucanases at 60°C (pH 5). Enzyme stability was maintained above 50% residual activity under ambient and refrigerated conditions, emphasizing its potential for field applications. This study provides a basis for developing sustainable enzyme bioformulations, contributing to environmentally friendly pest control solutions that reduce dependence on agrochemicals while promoting food safety and environmental health.