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dc.contributor.author
Garbus, Ingrid
dc.contributor.author
Carrera, Alicia
dc.contributor.author
Dubcovsky, Jorge
dc.contributor.author
Echenique, Carmen Viviana
dc.date.available
2017-10-19T17:56:31Z
dc.date.issued
2009-04
dc.identifier.citation
Garbus, Ingrid; Carrera, Alicia; Dubcovsky, Jorge; Echenique, Carmen Viviana; Physical mapping of durum wheat lipoxygenase genes; Elsevier; Journal of Cereal Science; 50; 1; 4-2009; 67-73
dc.identifier.issn
0733-5210
dc.identifier.uri
http://hdl.handle.net/11336/26822
dc.description.abstract
A bright yellow color is an important quality criterion for pasta making. Yellow color depends on the amount of carotenoid pigments in grain, which is the result of the balance between pigment synthesis and degradation by lipoxygenases (LPX). The organization of genes coding for lipoxygenases in the tetraploid wheat genome is not completely understood. Here, we report the screening of a durum wheat BAC library with barley probes to characterize the physical distribution of Lpx genes. PCR characterization and BAC fingerprinting of the positive clones suggests that Lpx-B1.1 and Lpx-B3 are less than 103-kb apart, whereas Lpx-B1.2 is further apart from them. In the A genome a partially deleted copy of Lpx-1 (Lpx-A1_like) was found, colocalizing within a 42 kbp region with Lpx-A3, confirming that in both genomes these two genes are close to each other. The knowledge of the physical location of these two genes is important to understand the evolution of this family but also has practical implications since closely linked genes are difficult to separate by recombination. This may limit the number of Lpx allele combinations that can be obtained and affect the selection of optimal Lpx allele combinations for pasta quality improvement.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Bac Library
dc.subject
Durum Wheat
dc.subject
Lipoxygenase Genes
dc.subject
Fingerprinting
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Physical mapping of durum wheat lipoxygenase genes
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-10-09T15:30:12Z
dc.journal.volume
50
dc.journal.number
1
dc.journal.pagination
67-73
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Garbus, Ingrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
dc.description.fil
Fil: Carrera, Alicia. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
dc.description.fil
Fil: Dubcovsky, Jorge. University of California at Davis; Estados Unidos
dc.description.fil
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
dc.journal.title
Journal of Cereal Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jcs.2009.02.008
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S073352100900040X


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