Capítulo de Libro

Starting a new recombinant protein production project in Escherichia coli

Título del libro: Methods in Enzymology. Recombinant Protein Expression: Prokaryotic Hosts and Cell-Free Systems

Aguilar Lucero, Dianela AilinIcon ; Cantoia, AlejoIcon ; Ceccarelli, Eduardo AugustoIcon ; Rosano, German LeandroIcon
Otros responsables: Kellman, Zvi; O´Dell, William
Fecha de publicación: 2021
Editorial: Elsevier
ISBN: 978-0-323-90146-8
Idioma: Inglés
Clasificación temática:

Resumen

One of the goals in recombinant protein production in Escherichia coli is to maximize productivity. High volumetric and specific yields can be reached after careful selection of expression strains and optimization of cultivation parameters. In this chapter, we review the many tools available to make the most out of this versatile microbial cell factory. Useful guidelines and options for troubleshooting production are presented.
Palabras clave: ESCHERICHIA COLI , RECOMBINANT PROTEINS , CULTIVATION FACTORS , INDUCER , PROTEIN AGGREGATION
 
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Tamaño: 658.1Kb
Formato: PDF
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Licencia
info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/266683
URL: https://www.sciencedirect.com/science/article/abs/pii/S0076687921003621
Colecciones
Capítulos de libros(IBR)
Capítulos de libros de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Citación
Aguilar Lucero, Dianela Ailin; Cantoia, Alejo; Ceccarelli, Eduardo Augusto; Rosano, German Leandro; Starting a new recombinant protein production project in Escherichia coli; Elsevier; 569; 2021; 3-18
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