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dc.contributor.author
dos Santos, Altair Brito  
dc.contributor.author
Larsen, Silas Dalum  
dc.contributor.author
Gomez Martinez, Carlos Daniel  
dc.contributor.author
Sørensen, Jakob Balslev  
dc.contributor.author
Perrier, Jean-François  
dc.date.available
2025-07-17T13:18:15Z  
dc.date.issued
2024-06  
dc.identifier.citation
dos Santos, Altair Brito; Larsen, Silas Dalum; Gomez Martinez, Carlos Daniel; Sørensen, Jakob Balslev; Perrier, Jean-François; Protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of neurodevelopmental encephalopathy; Elsevier; STAR Protocols; 5; 2; 6-2024; 1-12  
dc.identifier.issn
2666-1667  
dc.identifier.uri
http://hdl.handle.net/11336/266410  
dc.description.abstract
Here, we present a protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of STXBP1 neurodevelopmental encephalopathy (Stxbp1hap). We describe steps for preparing brain slices, positioning electrodes, and performing an excitability test to investigate microcircuit failures. This protocol is based on recording layer 2/3 cortical pyramidal neurons in response to stimulation of two independent sets of excitatory axons that recruit feedforward inhibition microcircuits.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
STXBP1  
dc.subject
Electrophysiology  
dc.subject
Microcircuits  
dc.subject
PV neurons  
dc.subject.classification
Neurociencias  
dc.subject.classification
Medicina Básica  
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of neurodevelopmental encephalopathy  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2025-07-14T10:35:54Z  
dc.journal.volume
5  
dc.journal.number
2  
dc.journal.pagination
1-12  
dc.journal.pais
Estados Unidos  
dc.description.fil
Fil: dos Santos, Altair Brito. Universidad de Copenhagen; Dinamarca  
dc.description.fil
Fil: Larsen, Silas Dalum. Universidad de Copenhagen; Dinamarca  
dc.description.fil
Fil: Gomez Martinez, Carlos Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina  
dc.description.fil
Fil: Sørensen, Jakob Balslev. Universidad de Copenhagen; Dinamarca  
dc.description.fil
Fil: Perrier, Jean-François. Universidad de Copenhagen; Dinamarca  
dc.journal.title
STAR Protocols  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2666166724001199  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.xpro.2024.102954  

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