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Artículo

Molecular basis of RNA recombination in the 3′UTR of chikungunya virus genome

Bardossy, Eugenia SoledadIcon ; Volpe, SebastianoIcon ; Suzuki, Yasutsugu; Merwaiss, FernandoIcon ; Faraj, Santiago EnriqueIcon ; Montes, Monica RaquelIcon ; Saleh, Maria Carla; Alvarez, Diego EzequielIcon ; Filomatori, Claudia VeronicaIcon
Fecha de publicación: 07/2024
Editorial: Oxford University Press
Revista: Nucleic Acids Research
ISSN: 0305-1048
e-ISSN: 1362-4962
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Virología

Resumen

Chikungunya virus (CHIKV) is a rapidly spreading re-emergent virus transmitted from mosquitoes to humans. The emergence of epidemic variants has been associated with changes in the viral genome, such as the duplication of repeated sequences in the 3 untranslated region (UTR).Indeed, blocks of repeated sequences seemingly favor RNA recombination, providing the virus with a unique ability to continuously change the 3´UTR architecture during host switching. In this work, we provide experimental data on the molecular mechanism of RNA recombination and describe specific sequence and structural elements in the viral 3´UTR that favor template switching of the viral RNA-dependent RNA polymerase on the 3´UTR. Furthermore, we found that a 3´UTR deletion mutant that exhibits markedly delayed replication in mosquito cells and impaired transmission in vivo, recombines in reference laboratory strains of mosquitoes. Altogether, our data provide novel experimental evidence indicating that RNA recombination can act as a nucleic acid repair mechanism to add repeated sequences that are associated to high viral fitness in mosquito during chikungunya virus replication.
Palabras clave: CHIKUNGUNYA , ALPHAVIRUS , RNA RECOMBINATION , VIRUS EVOLUTION
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/265244
URL: https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae650/7720764
DOI: http://dx.doi.org/10.1093/nar/gkae650
Colecciones
Articulos (IIBIO)
Articulos de INSTITUTO DE INVESTIGACIONES BIOTECNOLOGICAS
Articulos(IQUIFIB)
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Citación
Bardossy, Eugenia Soledad; Volpe, Sebastiano; Suzuki, Yasutsugu; Merwaiss, Fernando; Faraj, Santiago Enrique; et al.; Molecular basis of RNA recombination in the 3′UTR of chikungunya virus genome; Oxford University Press; Nucleic Acids Research; 52; 16; 7-2024; 9727-9744
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