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Artículo

Estimation of Band Broadening in Size Exclusion Chromatography. I. Theoretical Method Based on Analyzing Narrow Standards with a Molar Mass-Sensitive Detector

Yossen, Mariana MatildeIcon ; Vega, Jorge RubenIcon ; Meira, Gregorio RaulIcon
Fecha de publicación: 12/2006
Editorial: Elsevier Science
Revista: Journal Of Chromatography - A
ISSN: 0021-9673
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ingeniería Química

Resumen

A method is proposed for estimating the (asymmetrical and non-uniform) band broadening function (BBF) in size-exclusion chromatography (SEC). The following data are required: the molar mass calibration and the concentration- and molar mass chromatograms of a set of narrow standards. In the narrow range of each standard, the BBF is uniform but skewed. Each uniform BBF is estimated through a nonlinear optimization procedure that compares one (of the two) measured chromatograms with its theoretical prediction based on the other chromatogram. The method is validated with numerical examples that simulate the analyses of narrow standards exhibiting log-normal and Poisson weight chain length distributions. The BBF can be assumed of arbitrary shape, or represented by an exponentially-modified Gaussian (EMG). From the uniform BBF estimate, the true polydispersity of the standard can be determined. The global non-uniform BBF is obtained by interpolation between a set of uniform BBFs covering a wide range of elution volumes.
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/26398
URL: http://www.sciencedirect.com/science/article/pii/S002196730601243X
DOI: http://dx.doi.org/10.1016/j.chroma.2006.06.062
Colecciones
Articulos(INTEC)
Articulos de INST.DE DES.TECNOL.PARA LA IND.QUIMICA (I)
Citación
Yossen, Mariana Matilde; Vega, Jorge Ruben; Meira, Gregorio Raul; Estimation of Band Broadening in Size Exclusion Chromatography. I. Theoretical Method Based on Analyzing Narrow Standards with a Molar Mass-Sensitive Detector; Elsevier Science; Journal Of Chromatography - A; 1128; 1-2; 12-2006; 171-180
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