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Artículo

Effects of heme oxygenase isozymes on Leydig cells steroidogenesis

Piotrkowski, BarbaraIcon ; Monzón, Casandra MargaritaIcon ; Pagotto, Romina María del LujánIcon ; Reche, Cecilia; Besio Moreno, Marcos AlbertoIcon ; Cymeryng, Cora BetrizIcon ; Pignataro, Omar PedroIcon
Fecha de publicación: 10/2009
Editorial: BioScientifica
Revista: Journal of Endocrinology
ISSN: 0022-0795
e-ISSN: 1479-6805
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

In the present study, we demonstrate the expression of heme oxygenase (HO) isozymes, HO-1 and HO-2 (listed as HMOX1 and HMOX2 in the MGI Database), in MA-10 Leydig tumor cells and its effect on steroidogenesis. The well-known HO inducer, hemin, increased both HO-1 and HO-2 protein levels and HO-specific activity. Induction of HO by hemin inhibited basal, hCG-, and dibutyryl cAMP (db-cAMP)-induced steroidogenesis in a reversible way. When we studied the effect of HO isozymes along the steroid synthesis, we found that steroidogenic acute regulatory protein levels were decreased, and the conversion of cholesterol to pregnenolone was inhibited by hemin treatment, with no changes in the content of cholesterol side-chain cleavage enzyme (P450scc). hCG and db-cAMP also stimulated the expression of HO-1 and HO-2, and HO enzymatic activity in MA-10 cells. Basal and hCG-stimulated testosterone synthesis was also inhibited by hemin in rat normal Leydig cells. Taken together, these results suggest that: i) at least one of HO products (presumably carbon monoxide) inhibits cholesterol transport to the inner mitochondrial membrane and Leydig cell steroidogenesis by binding to the heme group of the cytochrome P450 enzymes, in a similar way as we described for nitric oxide, and ii) hCG stimulation results in the induction of an antioxidant enzymatic system (HO) acting as a cytoprotective mechanism in Leydig cells, as already demonstrated in the adrenal gland.
Palabras clave: Heme Oxygenase , Steroidogenesis , Leydig Cells
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/26331
URL: http://joe.endocrinology-journals.org/content/203/1/155.long
DOI: http://dx.doi.org/10.1677/JOE-09-0061
Colecciones
Articulos(CEFYBO)
Articulos de CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Citación
Piotrkowski, Barbara; Monzón, Casandra Margarita; Pagotto, Romina María del Luján; Reche, Cecilia; Besio Moreno, Marcos Alberto; et al.; Effects of heme oxygenase isozymes on Leydig cells steroidogenesis; BioScientifica; Journal of Endocrinology; 203; 1; 10-2009; 155-165
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