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dc.contributor.author
Matzkin, Maria Eugenia
dc.contributor.author
Yamashita, Soichi
dc.contributor.author
Ascoli, Mario
dc.date.available
2015-11-02T16:36:36Z
dc.date.issued
2013-03-07
dc.identifier.citation
Matzkin, Maria Eugenia; Yamashita, Soichi; Ascoli, Mario; The ERK1/2 pathway regulates testosterone synthesis by coordinately regulating the expression of steroidogenic enzymes in Leydig cells; Elsevier Ireland; Molecular And Cellular Endocrinology; 370; 1-2; 7-3-2013; 130-137
dc.identifier.issn
0303-7207
dc.identifier.uri
http://hdl.handle.net/11336/2629
dc.description.abstract
Adult mice with a Leydig cell specific deletion of MAPK kinase (MEK) 1 and 2 (Mek1(f)(/)(f);Mek2(-/-);Cre(+)) mice display Leydig cell hypoplasia and hypergonadotropic hypogonadism. We used radioimmunoassays and quantitative PCR to evaluate the function and expression of the Leydig cell genes involved in the conversion of cholesterol to testosterone (Star, Cyp11a1, Hsd3b6, Cyp17a1 and Hsd17b3), androgen metabolism (Srda1 and Dhrs9), and four transcription factors (Creb1, Nr5a1, Nr4a1 and Nr0b1) that regulate the expression of steroidogenic genes. We show that Star, Hsd3b6, Cyp17a1 and Hsd17b3 are downregulated in Ledyig cells of adult Mek1(f)(/)(f);Mek2(-/-);Cre(+) mice whereas Srda1 and Dhrs9 are upregulated and Creb1, Nr5a1, Nr4a1 and Nr0b1 are unchanged or upregulated. Functionally, all the downregulated genes but none of the upregulated genes contribute to the decrease in testosterone synthesis in Leydig cells of adult Mek1(f)(/)(f);Mek2(-/-);Cre(+) mice because they produce low testosterone and dihydrotestosterone when stimulated with hCG or when incubated with testosterone precursors such as progesterone or androstenedione.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Ireland
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
ANDROGENS
dc.subject
LEYDIG CELLS
dc.subject
LUTEINIZING HORMONE
dc.subject
MAP KINASE (ERK)
dc.subject
MAPK KINASE (MEK)
dc.subject
STEROIDOGENESIS
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
The ERK1/2 pathway regulates testosterone synthesis by coordinately regulating the expression of steroidogenic enzymes in Leydig cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.identifier.eissn
1872-8057
dc.journal.volume
370
dc.journal.number
1-2
dc.journal.pagination
130-137
dc.journal.pais
Irlanda
dc.journal.ciudad
Limerick
dc.description.fil
Fil: Matzkin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Yamashita, Soichi. University of Iowa; Estados Unidos
dc.description.fil
Fil: Ascoli, Mario. University of Iowa; Estados Unidos
dc.journal.title
Molecular And Cellular Endocrinology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.mce.2013.02.017
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0303720713000750
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