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dc.contributor.author
Sacca, Paula Alejandra
dc.contributor.author
Pistone Creydt, Virginia
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Tesone, Amelia Julieta
dc.contributor.author
Calvo, Juan Carlos
dc.date.available
2017-10-09T20:54:40Z
dc.date.issued
2009-12
dc.identifier.citation
Sacca, Paula Alejandra; Pistone Creydt, Virginia; Tesone, Amelia Julieta; Calvo, Juan Carlos; A novel and alternative in vitro method using microwave to study the epithelial-stromal interactions; Academic Journals; Scientific Research and Essay; 4; 12; 12-2009; 1487-1495
dc.identifier.issn
1992-2248
dc.identifier.uri
http://hdl.handle.net/11336/26290
dc.description.abstract
The goal of the present work was to obtain a simple and reproducible experimental model that would maintain the characteristics of the extracellular physiological environment of breast epithelial cells, both in factors as well as stromal structure, on which we could grow and evaluate changes of normal and tumor breast cells. 3T3-L1 pre-adipocytes (breast stromal cell model) were cultured and irradiated in a microwave oven at different times and potencies. In order to lose their proliferation ability, cells had to be irradiated twice at 650 Watts with a two-minute pulse each. The characteristics of the treated stromal support were analyzed for cell morphology, presence of DNA and proteins. We then evaluated on this support the effect on proliferation and migration of both normal and tumor - murine and human - breast epithelial cells. Both cell types increased their proliferation, while only tumor cells increased migration, thus improving their metastatic capacity. We believe this is a new and simple experimental method of studying epithelial-stromal cell interaction.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Journals
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Cancer
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Epithelial-Stromal Interactions
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Extracellular Matrix
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Microwave Oven
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
A novel and alternative in vitro method using microwave to study the epithelial-stromal interactions
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-10-04T15:03:34Z
dc.identifier.eissn
1992-2248
dc.journal.volume
4
dc.journal.number
12
dc.journal.pagination
1487-1495
dc.journal.pais
Kenia
dc.journal.ciudad
Nairobi
dc.description.fil
Fil: Sacca, Paula Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Pistone Creydt, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Tesone, Amelia Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.journal.title
Scientific Research and Essay
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.academicjournals.org/journal/SRE/article-abstract/E7782C819974
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