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dc.contributor.author
Silberstein, Claudia Marcela
dc.contributor.author
Pistone Creydt, Virginia
dc.contributor.author
Gerhardt, Elizabeth
dc.contributor.author
Nuñez, Pablo Alfredo
dc.contributor.author
Ibarra, Cristina Adriana
dc.date.available
2017-10-09T20:35:57Z
dc.date.issued
2008
dc.identifier.citation
Silberstein, Claudia Marcela; Pistone Creydt, Virginia; Gerhardt, Elizabeth; Nuñez, Pablo Alfredo; Ibarra, Cristina Adriana; Inhibition of water absorption in human proximal tubular epithelial cells in response to Shiga toxin-2; Springer; Pediatric Nephrology; 23; 11; -1-2008; 1981-1990
dc.identifier.issn
0931-041X
dc.identifier.uri
http://hdl.handle.net/11336/26282
dc.description.abstract
Postdiarrhea hemolytic uremic syndrome (HUS) is the most common cause of acute renal failure in children in Argentina. It is well established that Shiga toxin type 2 (Stx2) causes direct damage to glomerular endothelial cells and tubular epithelial cells, leading to a reduction in the water handling capacity of the kidney. In this study, we demonstrate that Stx2 and its B subunit (Stx2B) were able to inhibit water absorption across human renal tubular epithelial cell (HRTEC) monolayers without altering the short circuit current and the (3)H-mannitol permeability. Quantitative evaluation of (14)C-inulin transport across HRTEC monolayers showed a similar transport rate both before and after HRTEC treatment with Stx2 that confirmed the integrity of the paracellular pathway. Furthermore, Stx2 produced significant protein synthesis inhibition of HRTEC at concentrations as low as 0.001 ng/ml and 1 h of incubation, whereas Stx2B did not modify it at concentrations as high as 10,000 ng/ml and 6 h of incubation. Our findings suggest that whereas the action of Stx2 appears to be caused mainly by the inhibition of protein synthesis mediated by the A subunit, the binding of Stx2B subunit to the Gb3 receptor may affect the membrane mechanisms related to water absorption. We speculate that inhibition of water absorption may occur in proximal tubular cells in vivo in response to Stx2 and may contribute to the early event of HUS pathogenesis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Diarrhea
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Hemolytic Uremic Syndrome
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Acute Renal Failure
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Cytotoxicity
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Kidney Tubules
dc.subject.classification
Bioquímica y Biología Molecular
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
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Patología
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Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Inhibition of water absorption in human proximal tubular epithelial cells in response to Shiga toxin-2
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-10-04T16:55:18Z
dc.identifier.eissn
1432-198X
dc.journal.volume
23
dc.journal.number
11
dc.journal.pagination
1981-1990
dc.journal.pais
Alemania
dc.journal.ciudad
Berlín
dc.description.fil
Fil: Silberstein, Claudia Marcela. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina
dc.description.fil
Fil: Pistone Creydt, Virginia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Gerhardt, Elizabeth. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina
dc.description.fil
Fil: Nuñez, Pablo Alfredo. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Ibarra, Cristina Adriana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Pediatric Nephrology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00467-008-0896-9
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info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00467-008-0896-9
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/18607643
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