Artículo
Riboproteome remodeling during quiescence exit in Saccharomyces cerevisiae
Solari, Clara Andrea
; Ortolá Martínez, María Clara
; Fernandez, Juan M.; Bates, Christian; Cueto, Gerardo Ruben
; Valacco, Maria Pia
; Morales Polanco, Fabián; Moreno, Silvia Margarita
; Rossi, Silvia Graciela
; Ashe, Mark P.; Portela, Paula







Fecha de publicación:
01/2024
Editorial:
Elsevier
Revista:
iScience
ISSN:
2589-0042
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The quiescent state is the prevalent mode of cellular life in most cells. Saccharomyces cerevisiae is a useful model for studying the molecular basis of the cell cycle, quiescence, and aging. Previous studies indicate that heterogeneous ribosomes show a specialized translation function to adjust the cellular proteome upon a specific stimulus. Using nano LC-MS/MS, we identified 69 of the 79 ribosomal proteins (RPs) that constitute the eukaryotic 80S ribosome during quiescence. Our study shows that the riboproteome is composed of 444 accessory proteins comprising cellular functions such as translation, protein folding, amino acid and glucose metabolism, cellular responses to oxidative stress, and protein degradation. Furthermore, the stoichiometry of both RPs and accessory proteins on ribosome particles is different depending on growth conditions and among monosome and polysome fractions. Deficiency of different RPs resulted in defects of translational capacity, suggesting that ribosome composition can result in changes in translational activity during quiescence.
Palabras clave:
CELL BIOLOGY
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IEGEBA)
Articulos de INSTITUTO DE ECOLOGIA, GENETICA Y EVOLUCION DE BS. AS
Articulos de INSTITUTO DE ECOLOGIA, GENETICA Y EVOLUCION DE BS. AS
Articulos(IQUIBICEN)
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Citación
Solari, Clara Andrea; Ortolá Martínez, María Clara; Fernandez, Juan M.; Bates, Christian; Cueto, Gerardo Ruben; et al.; Riboproteome remodeling during quiescence exit in Saccharomyces cerevisiae; Elsevier; iScience; 27; 1; 1-2024; 1-23
Compartir
Altmétricas