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dc.contributor.author Phakachoed, N.
dc.contributor.author Suksombat, W.
dc.contributor.author Colombatto, Dario
dc.contributor.author Beauchemin, K. A.
dc.date.available 2017-10-06T20:20:57Z
dc.date.issued 2013-07
dc.identifier.citation Phakachoed, N.; Suksombat, W.; Colombatto, Dario; Beauchemin, K. A.; Use of fibrolytic enzymes additives to enhance in vitro ruminal fermentation of corn silage; Elsevier Science; Livestock Science; 157; 1; 7-2013; 100-112
dc.identifier.issn 1871-1413
dc.identifier.uri http://hdl.handle.net/11336/26173
dc.description.abstract Two experiments were conducted to evaluate the effect of four enzyme additives on ruminal fermentation of corn silage using a 48 h batch culture in vitro assay with buffer and ruminal fluid. Experiment 1 (Exp. 1) and Experiment 2 (Exp. 2) were conducted as completely randomized designs each with two runs and four replicates. The enzyme additives (E1, E2, E3, and E4) were commercial products that provided a range in endoglucanase, exoglucanase, and xylanase activities. For both xylanase (birch wood and oat spelt substrate) and endoglucanase (carboxymethylcellulose substrate), the enzyme products (per ml) were ranked E4>E1>E2>E3. In Exp. 1, the four enzymes were added at 0, 2, 4, and 8 μl/g of corn silage dry matter (DM), whereas in Exp. 2 enzymes were added at 0, 0.5, 1, 2, and 4 μl/g DM. Gas production (GP) was measured at 3, 6, 12, 18, 24, and 48 h after incubation. Disappearance of DM (DMD), neutral detergent fiber (NDFD), and acid detergent fiber (ADFD), and volatile fatty acid concentrations (VFA; total and individual molar proportions) were determined after 24 and 48 h. In Exp. 1, E1 and E2 had higher NDFD and ADFD at 24 and 48 h of incubation (P<0.001) compared with E3 and E4. Increasing dose rate increased NDFD and ADFD for all enzymes (except ADFD for E4 at 48 h), with the optimum dose rate dependant on the enzyme additive (dose×enzyme; P<0.01). There were some treatment effects on DMD and total GP at 24 and 48 h, but these responses were not consistent with responses in NDFD and ADFD. Experiment 2 was conducted to confirm the effects and optimum dose rate of each enzyme additive. In Exp. 2, DMD was not affected by enzyme after 24 and 48 h incubation. There were no enzyme×dose interactions for DMD, NDFD, or ADFD after 24 or 48 h of incubation (except for ADFD at 48 h). After 24 h, DMD, NDFD, and ADFD increased linearly with increasing dose (P<0.05); after 48 h DMD increased linearly, whereas NDFD increased quadratically with increasing enzyme dose (P<0.05). The ADFD increased linearly after 48 h for E3 and E4, but after 48 h ADFD increased quadratically for E1 and E2. Total GP was consistently lowest for E4 at both incubation times (P<0.05). There were no enzyme×dose interactions (P>0.05) for any of the fermentation variables at either 24 or 48 h of incubation in Exp. 2. There were differences amongst the additives for total VFA at 24 and 48 h (P≤0.05); increasing enzyme dose decreased total VFA after 24 h but increased total VFA at 48 h, such that all doses were higher than the control (P<0.001). Overall, the enzyme additives increased NDFD and ADFD of corn silage in vitro; however, E1 and E2 were more effective than E3 or E4. Responses to increasing dose of enzyme were generally linear or curvilinear, and the optimum dose rate differed amongst the products evaluated. Evaluation of the enzymes at 24 and 48 h generally led to the same ranking of the additives, and the degradation of NDF and ADF was more useful in differentiating the enzymes compared with DM and total GP.
dc.format application/pdf
dc.language.iso eng
dc.publisher Elsevier Science
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject EXOGENOUS FIBROLYTIC ENZYME
dc.subject CORN SILAGE
dc.subject DISAPPEARANCE
dc.subject IN VITRO BATCH CULTURE
dc.subject.classification Otras Producción Animal y Lechería
dc.subject.classification Producción Animal y Lechería
dc.subject.classification CIENCIAS AGRÍCOLAS
dc.title Use of fibrolytic enzymes additives to enhance in vitro ruminal fermentation of corn silage
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-09-19T14:23:56Z
dc.journal.volume 157
dc.journal.number 1
dc.journal.pagination 100-112
dc.journal.pais Países Bajos
dc.journal.ciudad Amsterdam
dc.description.fil Fil: Phakachoed, N.. Suranaree University of Technology; Tailandia
dc.description.fil Fil: Suksombat, W.. Suranaree University of Technology; Tailandia
dc.description.fil Fil: Colombatto, Dario. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Beauchemin, K. A.. Agriculture and Agri-Food Canada; Canadá
dc.journal.title Livestock Science
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.livsci.2013.06.020
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1871141313002837


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)