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Artículo

Lab on a chip (LOC) platform for drug screening against the intracellular forms of Trypanosoma cruzi

Cadena, María Fernand; Rosero Yánez, Gustavo IvanIcon ; Isa Jara, Ramiro; Belaunzarán, María LauraIcon ; Giulianotti, Marc A.; Pinilla, Clemencia; Alba Soto, Catalina DirneyIcon ; Perez, Maximiliano SebastianIcon ; Lerner, BetianaIcon ; Gimenez, GuadalupeIcon
Fecha de publicación: 05/2024
Editorial: Elsevier Science
Revista: Microchemical Journal
ISSN: 0026-265X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Celular, Microbiología

Resumen

Trypanosoma cruzi is the etiological agent of Chagas disease, endemic in Latin America with recent spreading to non-endemic areas due to human migration. The low efficacy and substantial prevalence of side effects of the approved medications benznidazole (BZN) and nifurtimox observed mainly during the chronic phase of Chagas disease, when most patients receive their diagnosis, demonstrate the need to find new therapeutic compounds to address this neglected global health disease. Here, we developed a platform for screening the antitrypanosomal activity of compounds over intracellular amastigotes using microfluidics, LOC devices, and advanced image analysis algorithms. This platform allows the simultaneous testing of six different concentrations of a compound and the automatic and high-precision processing of the images obtained during the experimental phase, providing quickly and easily a multitude of statistical data for the screening of new drugs. To assess the developed platform, we analyzed the effects of BZN as a reference drug and compared the dose–response curves with those obtained with a flow cytometry-based method, obtaining similar IC50 results. Furthermore, a collection of compounds obtained by chemical synthesis from a combinatorial library was tested over intracellular amastigotes in infected Vero cells.
Palabras clave: CHAGAS DISEASE , DRUG SCREENING , IMAGE ANALYSIS , MICROSCOPY
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/261131
URL: https://linkinghub.elsevier.com/retrieve/pii/S0026265X24009822
DOI: http://dx.doi.org/10.1016/j.microc.2024.110870
Colecciones
Articulos(IMPAM)
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Cadena, María Fernand; Rosero Yánez, Gustavo Ivan; Isa Jara, Ramiro; Belaunzarán, María Laura; Giulianotti, Marc A.; et al.; Lab on a chip (LOC) platform for drug screening against the intracellular forms of Trypanosoma cruzi; Elsevier Science; Microchemical Journal; 203; 5-2024; 1-10
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