Artículo
Analysis of DNA structure and sequence requirements for Pseudomonas aeruginosa MutL endonuclease activity
Correa, Elisa María Eugenia
; de Tullio, Luisina
; Vélez, Pablo S.; Martina, Mariana Andrea
; Argaraña, Carlos Enrique
; Barra, Jose Luis
Fecha de publicación:
08/2013
Editorial:
Oxford University Press
Revista:
Journal Of Biochemistry
ISSN:
0021-924X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The hallmark of the mismatch repair system in bacterial and eukaryotic organisms devoid of MutH is the presence of a MutL homologue with endonuclease activity. The aim of this study was to analyse whether different DNA structures affect Pseudomonas aeruginosa MutL (PaMutL) endonuclease activity and to determine if a specific nucleotide sequence is required for this activity. Our results showed that PaMutL was able to nick covalently closed circular plasmids but not linear DNA at high ionic strengths, while the activity on linear DNA was only found below 60 mM salt. In addition, single strand DNA, ss/ds DNA boundaries and negatively supercoiling degree were not required for PaMutL nicking activity. Finally, the analysis of the incision sites revealed that PaMutL, as well as Bacillus thuringiensis MutL homologue, did not show DNA sequence specificity.
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Articulos(CIQUIBIC)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Citación
Correa, Elisa María Eugenia; de Tullio, Luisina; Vélez, Pablo S.; Martina, Mariana Andrea; Argaraña, Carlos Enrique; et al.; Analysis of DNA structure and sequence requirements for Pseudomonas aeruginosa MutL endonuclease activity; Oxford University Press; Journal Of Biochemistry; 154; 6; 8-2013; 505-511
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