Characterization of cryopreserved and lyophilized Amniotic Membrane allograft

Abstract

The amniotic membrane is a tissue with characteristics and mechanisms of action evidenced by its clinical effects and through studies on its structural and biochemical composition, that turns it a useful tool for the treatment of pathologies and in tissue engineering. The Laboratorio de Hemoderivados (UNC) produces cryopreserved (MAHC) and lyophilized and irradiated (MAHLI) human amniotic membrane allografts with a good biocompatibility profile. The aim of this work was to characterize the composition and cell viability of allografts preserved by two different methods. The results showed that both conservation methods allow obtaining allografts with a high collagen content within the total protein content, resistance to collagenase and with the absence of viable cells.