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Artículo

Characterization of a modified gold platform for the development of a label-free anti-thrombin aptasensor

Jalit, YamileIcon ; Gutierrez, Fabiana AndreaIcon ; Dubacheva, Galina; Goyer, Cedric; Coche Guerente, Liliane; Defrancq, Eric; Labbe, Pierre; Rivas, Gustavo AdolfoIcon ; Rodriguez, Marcela CeciliaIcon
Fecha de publicación: 09/2012
Editorial: Elsevier Science
Revista: Biosensors & Bioelectronics
ISSN: 0956-5663
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Químicas

Resumen

This work reports the characterization of a modified gold surface as a platform for the development of a label free aptasensor for thrombin detection. The biorecognition platform was obtained by the self-assembly of 4-mercaptobenzoic acid onto a gold surface, covalent attachment of streptavidin and further immobilization of the biotinylated anti-thrombin aptamer. The biosensing platform was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring. The biorecognition event aptamer-thrombin was detected from changes in the SPR angle produced as a consequence of the molecular interaction between the aptasensor and the target protein. The biosensing platform demonstrated to be highly selective for human thrombin even in the presence of large excess of bovine thrombin, bovine serum albumin, cytochrome C, lysozyme and myoglobin. The relationship between the changes in the SPR angle and thrombin concentration was linear up to 0.19 μmol L−1 (R2=0.992) while the detection limit was of 12.0 nmol L−1 (240 fmol in the sample). This new sensing approach represents an interesting and promising alternative for the SPR-based quantification of thrombin.
Palabras clave: Thrombin Binding Aptamer , Aptasensor , Optical Aptasensor , Thrombin Biosensor , Protein Detection , Spr Sensing
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/26000
DOI: http://dx.doi.org/10.1016/j.bios.2012.08.061
URL: http://www.sciencedirect.com/science/article/pii/S0956566312006021
Colecciones
Articulos(INFIQC)
Articulos de INST.DE INVESTIGACIONES EN FISICO- QUIMICA DE CORDOBA
Citación
Jalit, Yamile; Gutierrez, Fabiana Andrea; Dubacheva, Galina; Goyer, Cedric; Coche Guerente, Liliane; et al.; Characterization of a modified gold platform for the development of a label-free anti-thrombin aptasensor; Elsevier Science; Biosensors & Bioelectronics; 41; 9-2012; 424-429
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