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dc.contributor.author
Diaz Gomez, Maria Isabel
dc.contributor.author
Fanelli, Silvia Laura
dc.contributor.author
Delgado, Aurora Maria
dc.contributor.author
Bietto, Florencia Matilde
dc.contributor.author
Castro, Jose Alberto
dc.contributor.author
Castro, Gerardo Daniel
dc.date.available
2017-10-05T18:08:51Z
dc.date.issued
2008-11
dc.identifier.citation
Diaz Gomez, Maria Isabel; Fanelli, Silvia Laura; Delgado, Aurora Maria; Bietto, Florencia Matilde; Castro, Jose Alberto; et al.; Deleterious effects induced by oxidative stress in liver nuclei from rats receiving an alcohol-containing liquid diet; Sage Publications Ltd; Toxicology And Industrial Health; 24; 10; 11-2008; 625-634
dc.identifier.issn
0748-2337
dc.identifier.uri
http://hdl.handle.net/11336/25990
dc.description.abstract
Highly purified rat-liver nuclei were previously shown to have nuclear ethanol (EtOH) metabolizing system able to bioactivate alcohol to acetaldehyde and 1-hydroxyethyl radicals. These reactive metabolites were able to covalently bind to nuclear proteins and lipids potentially being able to provoke oxidative stress of nuclear components. In this study, the above-mentioned possibility was explored. Sprague Dawley male rats (125–150 g) were fed a standard Lieber and De Carli liquid diet for 28 days. Controls were pair-fed with a diet, in which EtOH was isocalorically replaced with carbohydrate. The presence of a chlorzoxazone hydroxylase activity inducible by the repetitive EtOH drinking further suggested the presence of CYP2E1 in the highly purified nuclei. Nuclei from EtOH-drinking rats evidenced significantly increased susceptibility to a t-butyl hydroperoxide challenge as detected by chemiluminescence emission, increased formation of protein carbonyls, and decreased content of protein sulfhydryls. In contrast, no significant changes in the nuclear lipid hydroperoxides formation or even decreases in the 8-oxo-7,8-dihydro-2-deoxyguanosine were observed. No significant differences were observed in different parameters of the alkaline Comet assay. In immunohistochemical studies performed, no expression of p53 was observed in the livers of the animals under the experimental conditions tested. Since nuclear proteins and lipids are known to play a role in cell growth, differentiation, repair and signaling, their alterations by either oxidative stress, or by covalent binding might be of relevance to liver tumor promotion.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Sage Publications Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Alcohol
dc.subject
Cyp2e1
dc.subject
Ethanol
dc.subject
Nuclei
dc.subject
Microsomes
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Deleterious effects induced by oxidative stress in liver nuclei from rats receiving an alcohol-containing liquid diet
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-26T18:59:46Z
dc.journal.volume
24
dc.journal.number
10
dc.journal.pagination
625-634
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Diaz Gomez, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina
dc.description.fil
Fil: Fanelli, Silvia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; Argentina
dc.description.fil
Fil: Delgado, Aurora Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina
dc.description.fil
Fil: Bietto, Florencia Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina
dc.description.fil
Fil: Castro, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina
dc.description.fil
Fil: Castro, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina
dc.journal.title
Toxicology And Industrial Health
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/10.1177/0748233708101207
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1177/0748233708101207
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