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Díaz, Alejandra Graciela  
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Soto, Pedro  
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Estein, Silvia Marcela  
dc.date.available
2025-04-25T10:14:53Z  
dc.date.issued
2011  
dc.identifier.citation
Serological crossreactivity in diagnosis of brucellosis in Balb/c mice following administration of bovine commercial vaccines containing Gram negative bacteria; Brucellosis 2011: International Research Conference; Ciudad Autónoma de Buenos Aires; Argentina; 2011; 116-116  
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http://hdl.handle.net/11336/259589  
dc.description.abstract
Control of Bovine Brucellosis in Argentina is based on vaccination of calves with B. abortus S19, and detection and slaughter of positive reactors. Screening tests for the diagnosis of this disease are BPA (Buffered Plate Antigen) and FPA (Fluorescence Polarization Assay). Both tests detect antibodies to the O-chain, which is the immunodominant epitope of LPS in smooth Brucella spp. and are susceptible to false positive results (1, 2). The aim of this work was to study cross reacting antibodies resulting from administration of commercial vaccines containing Gram negative bacteria that share antigenic determinants with Brucella spp. in the mouse model. Female BALB/c mice 6-8 weeks old were randomly distributed in nine groups (n=5) and vaccinated using different commercial immunogens. Booster was performed at 25 day. In addition, two positive control groups immunized with B. abortus S19 (n=6) and infected with B. abortus S544 (n=5) were included. Blood samples were obtained at 0, 7, 15, 40, 50 and 60 days after immunization. Sera were analyzed by BPA and FPA tests. Immunized mice with commercial vaccines gave positive results to BPA at different points during the experiment, but at the end all mice were negative. None of sera from vaccinated mice reacted in FPA. Mice from positive control groups remained positive to BPA throughout the experiment. Infected mice were positive to FPA while mice from B. abortus vaccine strain had variable results. Cross reaction antibodies were induced by vaccines containing mainly Pasteurella haemolytica (Mannhemia haemolytica), P. multocida, Haemophilus somnus (Histophilus somni), Campylobacter fetus fetus and Moraxella bovis. These results showed that certain bovine commercial vaccines were able to induce non specific serological reactions in conventional serological test of bovine brucellosis (BPA), but these reactions were observed for a variable period. False positive reactions can be minimized by using other tests such us FPA. This technique showed to be a reliable test to overcome occurrence of serological cross reactions in bovine brucellosis diagnosis (3).  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Asociación Argentina de Microbiología  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
SEROLOGICAL CROSSREACTIVITY  
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BRUCELLA ABORTUS  
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MICE  
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BPA  
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FPA  
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COMMERCIAL VACCINES  
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Ciencias Veterinarias  
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Ciencias Veterinarias  
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CIENCIAS AGRÍCOLAS  
dc.title
Serological crossreactivity in diagnosis of brucellosis in Balb/c mice following administration of bovine commercial vaccines containing Gram negative bacteria  
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info:eu-repo/semantics/publishedVersion  
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info:eu-repo/semantics/conferenceObject  
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info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2025-04-09T13:48:52Z  
dc.journal.pagination
116-116  
dc.journal.pais
Argentina  
dc.journal.ciudad
Ciudad Autónoma de Buenos Aires  
dc.description.fil
Fil: Díaz, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina  
dc.description.fil
Fil: Soto, Pedro. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina  
dc.description.fil
Fil: Estein, Silvia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina  
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Autor  
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Autor  
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Autor  
dc.coverage
Internacional  
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Conferencia  
dc.description.nombreEvento
Brucellosis 2011: International Research Conference  
dc.date.evento
2011-09-21  
dc.description.ciudadEvento
Ciudad Autónoma de Buenos Aires  
dc.description.paisEvento
Argentina  
dc.type.publicacion
Book  
dc.description.institucionOrganizadora
Asociación Argentina de Microbiología  
dc.source.libro
Abstracts: Brucellosis 2011. International Research Conference  
dc.date.eventoHasta
2011-09-23  
dc.type
Conferencia