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dc.contributor.author
Díaz, Alejandra Graciela

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Soto, Pedro

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Estein, Silvia Marcela

dc.date.available
2025-04-25T10:12:42Z
dc.date.issued
2011
dc.identifier.citation
Non specific serological reactions in BPA and FPA tests following immunization with inactivated Gram negative bacteria in the mouse model; Brucellosis 2011: International Research Conference.; Ciudad Autónoma de Buenos Aires; Argentina; 2011; 116-116
dc.identifier.uri
http://hdl.handle.net/11336/259588
dc.description.abstract
Most serological test in diagnosis of bovine brucellosis are based on detection of antibodies to the O-chain (OPS) epitopes of LPS (2). Thus, non specific serological reactions are observed between Brucella spp. and other Gram (-) bacteria that share antigenic determinants in the OPS molecule (1,3). The aim of this study was to determine cross reacting antibodies resulting from the administration of different inactivated Gram (-) bacteria in the mouse model. Female BALB/c mice 6-8 weeks old were randomly distributed into five groups. Following bacteria were used: (I) Campylobacter fetus fetus (n=4), (II) Haemophilus somnus (Histophilus somni) (n=4), (III) Moraxella bovis (n=5), (IV) Pasteurella haemolytica (Mannhemia haemolytica) (n=4) and (V) Pasteurella multocida (n=5). Bacteria were cultured, inactivated, formulated in Incomplete Freund Adjuvant and employed as immunogens. Booster was carried out at day 25. A positive control group infected with B. abortus S544 was also included. Sera samples were obtained before immunization and at weeks 2, 4, 5, 6, 7, 9, 12, 15, 18, 21, 24 and 27 post vaccination. Buffered Plate Antigen (BPA) and Fluorescence Polarization Assay (FPA) were performed on all specimens. All mice had negative results in both tests before vaccination. Hundred percent of animals gave positive result at week 2 (group V), week 4 (groups II, III and IV) and at week 6 (group I) to BPA. At the end of the experiment, all animals in group V (P. multocida) remained positive to this test while in the other groups only one mouse per group reacted to BPA. In contrast, only sera from groups IV (Pasteurella haemolytica) and V (Pasteurella multocida) were positive to FPA. The highest number and most persistent reactions were found in mice immunized with P. multocida (from 80% reactors at week 4 to 20% at the end of trial). Mice from positive control group remained positive to both tests throughout the experiment. These results showed that Gram (-) bacteria included in the experiment induced cross reacting antibodies with B. abortus. However, differences in cross reactivity between bacterial genera were observed. Although false positive reactions were found in both tests, FPA was more reliable. In conclusion, Gram (-) bacteria can be a potential source of confusion in serological diagnosis of bovine brucellosis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Asociación Argentina de Microbiología

dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
SEROLOGICAL INTERFERENCE
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BRUCELLA ABORTUS
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GRAMM NEGATIVE
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BPA
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FPA
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MICE
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Ciencias Veterinarias

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Ciencias Veterinarias

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CIENCIAS AGRÍCOLAS

dc.title
Non specific serological reactions in BPA and FPA tests following immunization with inactivated Gram negative bacteria in the mouse model
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2025-04-09T13:48:41Z
dc.journal.pagination
116-116
dc.journal.pais
Argentina

dc.journal.ciudad
Ciudad Autónoma de Buenos Aires
dc.description.fil
Fil: Díaz, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina
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Fil: Soto, Pedro. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina
dc.description.fil
Fil: Estein, Silvia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina
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Autor

dc.conicet.rol
Autor

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Autor

dc.coverage
Internacional
dc.type.subtype
Conferencia
dc.description.nombreEvento
Brucellosis 2011: International Research Conference.
dc.date.evento
2011-09-21
dc.description.ciudadEvento
Ciudad Autónoma de Buenos Aires
dc.description.paisEvento
Argentina

dc.type.publicacion
Book
dc.description.institucionOrganizadora
Asociación Argentina de Microbiología
dc.source.libro
Abstracts: Brucellosis 2011. International Research Conference
dc.date.eventoHasta
2011-09-23
dc.type
Conferencia
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