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dc.contributor.author Marin Briggiler, Clara Isabel
dc.contributor.author Veiga, Maria Florencia
dc.contributor.author Matos, María Laura
dc.contributor.author González Echeverría, M. F.
dc.contributor.author Furlong, L. I.
dc.contributor.author Vazquez, Monica Hebe
dc.date.available 2017-10-04T19:35:02Z
dc.date.issued 2008-12
dc.identifier.citation Marin Briggiler, Clara Isabel; Veiga, Maria Florencia; Matos, María Laura; González Echeverría, M. F.; Furlong, L. I.; et al.; Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization; Oxford University Press; Molecular Human Reproduction; 14; 10; 12-2008; 561-571
dc.identifier.issn 1360-9947
dc.identifier.uri http://hdl.handle.net/11336/25920
dc.description.abstract Epithelial cadherin (E-cadherin) has been involved in several calcium-dependent cell-cell adhesion events; however, its participation in gamete interaction has not been fully investigated. Our results have demonstrated expression of E-cadherin mRNA in the human male reproductive tract showing higher levels in the caput, corpus and cauda epididymis than in the testis. The mature 122 kDa E-cadherin was detected in epididymal protein extracts and was localized in the epithelial cells from the three epididymal regions. Moreover, the 86 kDa E-cadherin ectodomain was found in cauda epididymal and seminal plasma. Western immunoblotting of human sperm protein extracts allowed the identification of four E-cadherin forms (122, 105, 97 and 86 kDa). The protein was localized in the acrosomal region of intact spermatozoa, remained associated with the head of acrosome-reacted cells and was also detected on the oocyte surface. A similar localization was determined for other proteins of the adhesion complex (beta-catenin and actin). Spermatozoa incubated with anti-E-cadherin antibodies showed impaired binding to homologous zona pellucida (ZP); in addition, presence of these antibodies inhibited the penetration of human spermatozoa to ZP-free hamster oocytes. The results presented here describe the expression of E-cadherin in the male reproductive tract and gametes and strongly suggest its involvement in adhesion events during human fertilization. The identification of proteins involved in gamete interaction will contribute to the understanding of the molecular basis of fertilization and help in the diagnosis and treatment of infertility.
dc.format application/pdf
dc.language.iso eng
dc.publisher Oxford University Press
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject HUMAN SPERMATOZOA
dc.subject EPITHELIAL CADHERIN
dc.subject FERTILIZATION
dc.subject OOCYTE
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-09-25T18:31:30Z
dc.identifier.eissn 1460-2407
dc.journal.volume 14
dc.journal.number 10
dc.journal.pagination 561-571
dc.journal.pais Reino Unido
dc.journal.ciudad Oxford
dc.description.fil Fil: Marin Briggiler, Clara Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires; Argentina
dc.description.fil Fil: Veiga, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires; Argentina
dc.description.fil Fil: Matos, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires; Argentina
dc.description.fil Fil: González Echeverría, M. F.. Fertilab; Argentina
dc.description.fil Fil: Furlong, L. I.. Instituto Hospital del Mar de Investigaciones Medicas; España
dc.description.fil Fil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires; Argentina
dc.journal.title Molecular Human Reproduction
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/molehr/article-lookup/doi/10.1093/molehr/gan053
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/molehr/gan053
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/pmid/18829448


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