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dc.contributor.author
Marin Briggiler, Clara Isabel
dc.contributor.author
Veiga, Maria Florencia
dc.contributor.author
Matos, María Laura
dc.contributor.author
González Echeverría, M. F.
dc.contributor.author
Furlong, L. I.
dc.contributor.author
Vazquez, Monica Hebe
dc.date.available
2017-10-04T19:35:02Z
dc.date.issued
2008-12
dc.identifier.citation
Marin Briggiler, Clara Isabel; Veiga, Maria Florencia; Matos, María Laura; González Echeverría, M. F.; Furlong, L. I.; et al.; Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization; Oxford University Press; Molecular Human Reproduction; 14; 10; 12-2008; 561-571
dc.identifier.issn
1360-9947
dc.identifier.uri
http://hdl.handle.net/11336/25920
dc.description.abstract
Epithelial cadherin (E-cadherin) has been involved in several calcium-dependent cell-cell adhesion events; however, its participation in gamete interaction has not been fully investigated. Our results have demonstrated expression of E-cadherin mRNA in the human male reproductive tract showing higher levels in the caput, corpus and cauda epididymis than in the testis. The mature 122 kDa E-cadherin was detected in epididymal protein extracts and was localized in the epithelial cells from the three epididymal regions. Moreover, the 86 kDa E-cadherin ectodomain was found in cauda epididymal and seminal plasma. Western immunoblotting of human sperm protein extracts allowed the identification of four E-cadherin forms (122, 105, 97 and 86 kDa). The protein was localized in the acrosomal region of intact spermatozoa, remained associated with the head of acrosome-reacted cells and was also detected on the oocyte surface. A similar localization was determined for other proteins of the adhesion complex (beta-catenin and actin). Spermatozoa incubated with anti-E-cadherin antibodies showed impaired binding to homologous zona pellucida (ZP); in addition, presence of these antibodies inhibited the penetration of human spermatozoa to ZP-free hamster oocytes. The results presented here describe the expression of E-cadherin in the male reproductive tract and gametes and strongly suggest its involvement in adhesion events during human fertilization. The identification of proteins involved in gamete interaction will contribute to the understanding of the molecular basis of fertilization and help in the diagnosis and treatment of infertility.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Oxford University Press
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Human Spermatozoa
dc.subject
Epithelial Cadherin
dc.subject
Fertilization
dc.subject
Oocyte
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-25T18:31:30Z
dc.identifier.eissn
1460-2407
dc.journal.volume
14
dc.journal.number
10
dc.journal.pagination
561-571
dc.journal.pais
Reino Unido
dc.journal.ciudad
Oxford
dc.description.fil
Fil: Marin Briggiler, Clara Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: Veiga, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: Matos, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: González Echeverría, M. F.. Fertilab; Argentina
dc.description.fil
Fil: Furlong, L. I.. Instituto Hospital del Mar de Investigaciones Medicas; España
dc.description.fil
Fil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires; Argentina
dc.journal.title
Molecular Human Reproduction
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/molehr/article-lookup/doi/10.1093/molehr/gan053
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/molehr/gan053
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/18829448


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