Phytotoxicity bioassays in cigarette butts leachates after treatments by white rot fungi

Name: Phytotoxicity bioassays in cigarette butts leachates after treatments by white rot fungi
License: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Keywords: SEED TOXICITY TEST

Datos de investigación

Phytotoxicity bioassays in cigarette butts leachates after treatments by white rot fungi

Autores: Núñez, María del Pilar Icon

; Butler, Matias Icon

Colaboradores: Calfayan, Laura Mariel Icon

; Itria, Raúl Fabio; Levin, Laura Noemi Icon

Publicador: Consejo Nacional de Investigaciones Científicas y Técnicas

Fecha de depósito: 16/04/2025

Fecha de creación: 2025

Clasificación temática:

Biotecnología Medioambiental; Micología; Ciencias Medioambientales

Resumen

The dataset includes a series of root and hypocotyl length measurements performed on Raphanus sativus seedlings employed for toxicity bioassays. Tested samples included cigarette butts (CBs) leachates obtained from the fungal treatments and untreated CBs at different growth times, i.e. weeks 0, 2, 4 and 6 (W0, W2, W4, and W6). Two independent control experiments (C1 and C2) using water were conducted along with tested samples from weeks 0, 2 and 4 (C1) and week 6 (C2).

Métodos

Four fungal strains of white rot fungi (BAFC 2034, 4765, 4766 and 4767) were grown in a medium of water-soaked cigarette butts in axenic conditions at Erlenmeyer-scale during six weeks, extracting leachate samples every 2 weeks (100 g CBs/L). R. sativus seeds were acquired from a local seed store (Semillería Duran), ensuring that they were free of fungicides or pesticides and presented optimal germination power and low variability in root and hypocotyl elongation. The bioassay methodology was adapted from the IRAM 29114 protocol. Briefly, the bioassay consisted in placing a disc of filter paper (90 mm diam.) in glass Petri dishes saturated with 5.0 mL of the tested samples, avoiding the formation of air pockets. In each Petri dish, ten seeds were deposited leaving enough space among them to allow root elongation. Then, Petri dishes were placed on a tray covered by a plastic bag to prevent moisture loss and light exposure during the test period, and incubated for 120 h at 22 ± 2 °C. Each sample tested was evaluated in triplicate. The number of seeds that germinated in each Petri dish was recorded, considering the visible appearance of the root as a germination criterion. The length of the root and hypocotyl of each of the seedlings was measured using a caliber.

Palabras clave: SEED TOXICITY TEST, SEED GERMINATION, ROOT AND HYPOCOTYL ELONGATION, TOBACCO

Alcance geográfico

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Identificador del recurso

URI: http://hdl.handle.net/11336/258948

Colecciones

Datos de Investigación (IIIA)
Datos de Investigación de INSTITUTO DE INVESTIGACION E INGENIERIA AMBIENTAL

Datos de Investigación(INMIBO (EX - PROPLAME))
Datos de Investigación de INSTITUTO DE MICOLOGIA Y BOTANICA

Citación

Núñez, María del Pilar; Butler, Matias; (2025): Phytotoxicity bioassays in cigarette butts leachates after treatments by white rot fungi. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/258948

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Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)