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Artículo

Driving forces for the adsorption of a His-tag Chagas antigen: a rational approach to design bio-functional surfaces

Valenti, Laura ElisaIcon ; Smania, AndreaIcon ; de Pauli, Carlos PrimoIcon ; Giacomelli, Carla EugeniaIcon
Fecha de publicación: 08/2013
Editorial: Elsevier Science
Revista: Colloids and Surfaces B: Biointerfaces
ISSN: 0927-7765
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Químicas

Resumen

In order to rationally design a bio-functional surface based on the adsorption of a His-tag antigen, three requirements have to be considered: the bio-recognition element, the driving forces for the adsorption process and the detection mode of the bio-recognition event. This work is focused on the study of the adsorption mechanism of the His-tag H49 Chagas antigen on Ni(II) modified substrates. In order to construct the bio-functional surface, the gen of the H49 Chagas antigen was modified to incorporate His6 moiety at the N-terminal (His6-H49). Then, its physical adsorption and bio-affinity interaction with the solid substrate was studied by reflectometry. Besides His-Ni(II) bio-affinity interactions, His6-H49 was also physically adsorbed on Ni(II) modified substrates, leading to randomly oriented antigens. These loosely attached bio-molecules were partially removed using conditions of electrostatic repulsion. On the other hand, bio-affinity interactions, resulting in site-oriented molecules on the substrate, were only removable by specific competitors for Ni(II) surface sites. Finally, the surface bio-activity was determined from the peak separations of voltammetry waves due to the change of the electron transfer kinetics of a redox probe through the bio-functional surface (working electrode).
Palabras clave: H49 Antigen , Ni(Ii) Modified Substrates , Adsorption Mechanism , Bio-Affinity Interaction , Immunosensor , Direct Non-Labeled Detection
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/25843
DOI: http://dx.doi.org/10.1016/j.colsurfb.2013.07.059
URL: http://www.sciencedirect.com/science/article/pii/S0927776513004955
Colecciones
Articulos(CIQUIBIC)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Articulos(INFIQC)
Articulos de INST.DE INVESTIGACIONES EN FISICO- QUIMICA DE CORDOBA
Citación
Valenti, Laura Elisa; Smania, Andrea; de Pauli, Carlos Primo; Giacomelli, Carla Eugenia; Driving forces for the adsorption of a His-tag Chagas antigen: a rational approach to design bio-functional surfaces; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 112; 8-2013; 294-301
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